Scalable Neuroanatomical and Behavioral Phenotyping of Radio Frequency Radiation on Young Zebrafish

IEEE Open J Eng Med Biol. 2024 Jun 28:6:89-99. doi: 10.1109/OJEMB.2024.3420247. eCollection 2025.

Abstract

Objective: In our wireless-centric world, evaluating the health effects of radio frequency electromagnetic radiation (RF-EMR) is crucial. An existing research gap pertains to the replication of real-world specific absorption rates (SAR) for RF-EMR, especially within aquatic environments. We aimed to bridge this gap using an innovative TEM cell platform to replicate realistic SAR conditions in water and assess RF-EMR's impact on neuroanatomical and behavioral changes. Results: We examined RF-EMR effects on zebrafish embryos exposed to RF-EMR during the 4-58 hours post-fertilization phase. Temporary neuroanatomical enlargements and minor behavioral shifts were observed, diminishing by day 8 post-fertilization. Conclusion: Short-term RF-EMR exposure at tested levels did not yield significant long-term effects. Nevertheless, investigating prolonged exposure remains imperative. Our study serves as a pioneering model for future investigations into the biological consequences of RF-EMR exposure, highlighting the importance of assessing its health implications in our wireless-centric world.

Keywords: Behavioral effects; electromagnetic exposure; high-throughput analysis; zebrafish.

Grants and funding

This work was supported by the Research Grants Council of the Hong Kong Special Administrative Region, and China under Project CityU 11217019.