Dactylogyrus pharyngocephalus is a dactylogyrid monogenean parasite originally described by Kulkarni, 1970 from tank goby Glossogobius giuris (Hamilton, 1822) in South India. The species has not been recorded since then. Fresh specimens of D. pharyngocephalus were collected from northeast India and examined morpho-molecularly. Temporary (glycerine mounted) and permanent (stained with Gomori's trichrome and DPX mounted) slides were made and photographed. Primer set C1 and D2 was employed to amplify a fragment of the 28S rRNA gene. The resulting nucleotide sequences, when examined by the BLAST tool, did not show 100% homology with any of the sequences deposited in GenBank. Based on new morpho-molecular data, the description of D. pharyngocephalus is revised, including the first description of its ventral bar.