Virome analysis unveils a rich array of newly identified viruses in the red swamp crayfish Procambarus clarkii

Guangyu Guo; Zhi Liu; Jiamin Zeng; Hongyu Yan; Gongrui Chen; Peiyun Han; Xinyi He; Dandan Zhou; Shaoping Weng; Jianguo He; Muhua Wang

doi:10.1016/j.virol.2024.110308

Virome analysis unveils a rich array of newly identified viruses in the red swamp crayfish Procambarus clarkii

Virology. 2024 Nov 16:601:110308. doi: 10.1016/j.virol.2024.110308. Online ahead of print.

Authors

Guangyu Guo¹, Zhi Liu¹, Jiamin Zeng², Hongyu Yan², Gongrui Chen², Peiyun Han², Xinyi He², Dandan Zhou¹, Shaoping Weng², Jianguo He³, Muhua Wang⁴

Affiliations

¹ School of Marine Sciences, State Key Laboratory of Biocontrol, Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), China-ASEAN Belt and Road Joint Laboratory on Mariculture Technology, Sun Yat-sen University, Zhuhai 519082, China.
² School of Life Sciences, Guangdong Province Key Laboratory for Aquatic Economic Animals, Sun Yat-sen University, Guangzhou 510275, China.
³ School of Marine Sciences, State Key Laboratory of Biocontrol, Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), China-ASEAN Belt and Road Joint Laboratory on Mariculture Technology, Sun Yat-sen University, Zhuhai 519082, China; School of Life Sciences, Guangdong Province Key Laboratory for Aquatic Economic Animals, Sun Yat-sen University, Guangzhou 510275, China. Electronic address: lsshjg@mail.sysu.edu.cn.
⁴ School of Marine Sciences, State Key Laboratory of Biocontrol, Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), China-ASEAN Belt and Road Joint Laboratory on Mariculture Technology, Sun Yat-sen University, Zhuhai 519082, China; School of Life Sciences, Guangdong Province Key Laboratory for Aquatic Economic Animals, Sun Yat-sen University, Guangzhou 510275, China. Electronic address: wangmuh@mail.sysu.edu.cn.

PMID: 39556981
DOI: 10.1016/j.virol.2024.110308

Abstract

The red swamp crayfish (Procambarus clarkii) is the second most widely cultured crustacean globally. As a highly invasive species with a worldwide distribution, P. clarkii presents a substantial risk for the transmission of viral pathogens to native aquatic organisms. Recently, the emergence of growth retardation disease (GRD) in P. clarkii has led to significant production declines and economic losses. A comprehensive viromic analysis could offer valuable insights into the potential viral pathogens harbored by P. clarkii. Here we systematically examined the RNA viromes of healthy and GRD-affected P. clarkii collected from Qianjiang, China. Our investigation identified a total of 1729 viral species across 21 known viral taxa, with 1603 species being previously unreported. The orders Picornavirales, Tolivirales, and Nodamuvirales were predominant in both species count and relative abundance. Moreover, seven viruses exhibited higher abundance in GRD-affected P. clarkii compared to healthy individuals. Our work uncovers an unexpectedly diverse RNA viral community within P. clarkii and identifies potential viral pathogens associated with GRD in this species.

Keywords: Crustacean; Growth retardation disease; Procambarus clarkii; Virome; Virus diversity.