Using amino acid waste liquid as functional supplement to change microbial community in up-flow anaerobic sludge blanket treatment of methanolic wastewater

Liuying Song; Juntong Ha; Shunan Zhao; Weiquan Li; Yu Qin; Qigui Niu; Ruiping Liu; Yu-You Li

doi:10.1016/j.biortech.2024.131825

Using amino acid waste liquid as functional supplement to change microbial community in up-flow anaerobic sludge blanket treatment of methanolic wastewater

Bioresour Technol. 2024 Nov 14:416:131825. doi: 10.1016/j.biortech.2024.131825. Online ahead of print.

Authors

Liuying Song¹, Juntong Ha², Shunan Zhao³, Weiquan Li², Yu Qin¹, Qigui Niu⁴, Ruiping Liu³, Yu-You Li⁵

Affiliations

¹ Department of Civil and Environmental Engineering, Graduate School of Engineering, Tohoku University, 6-6-06 Aza, Aramaki, Aoba-ku, Sendai, Miyagi 980-8579, Japan.
² Department of Frontier Science for Advanced Environment, Graduate School of Environmental Studies, Tohoku University, 6-6-20 Aoba, Aramaki-Aza, Aoba-ku, Sendai, Miyagi 980-8579, Japan.
³ Center for Water and Ecology, State Key Joint Laboratory of Environment Simulation and Pollution Control, School of Environment, Tsinghua University, Beijing 100084, China.
⁴ School of Environmental Science and Engineering, Shandong University, 72#Jimo Binhai Road, Qingdao, Shandong Province 266237, China.
⁵ Department of Civil and Environmental Engineering, Graduate School of Engineering, Tohoku University, 6-6-06 Aza, Aramaki, Aoba-ku, Sendai, Miyagi 980-8579, Japan; Department of Frontier Science for Advanced Environment, Graduate School of Environmental Studies, Tohoku University, 6-6-20 Aoba, Aramaki-Aza, Aoba-ku, Sendai, Miyagi 980-8579, Japan. Electronic address: gyokuyu.ri.a5@tohoku.ac.jp.

PMID: 39547297
DOI: 10.1016/j.biortech.2024.131825

Abstract

In this study, amino acid waste liquid was employed as a functional supplement (designated as amino acid-rich FS) in the up-flow anaerobic sludge blanket (UASB) treatment of methanolic wastewater. The effect of amino acid-rich FS was evaluated through repeated batch tests, showing that a 0.5% and 1% dosage increased the maximum methane production rate by 93.60% and 123.04%, respectively, by promoting faster methanol degradation. Additionally, long-term operation of the UASB reactor was conducted with increased dosages of amino acid-rich FS, resulting in improved performance. Microbial community analysis demonstrated that the addition of amino acid-rich FS enhanced microbial diversity, with the abundance of Sporomusa increasing by 47.5 times. Beyond the original cooperative relationships, an additional synergy between Sporomusa and Methanosarcina was observed. These findings could address the key challenge of limited microbial diversity in the anaerobic treatment of methanolic wastewater.

Keywords: Amino acid-rich FS; Methanol utilization; Microbial synergy; UASB performance.