This study explores the impact of flavonols (kaempferol, quercetin, kaempferitrin, and isorhamnetin) on the gel properties of myosin derived from silver carp (Hypophthalmichthys molitrix). Before employing a two-stage heating process, myosin samples are pretreated with flavonols, which enhance the structural stability of myosin and aid in the dispersion of myosin molecules. These pretreatments prepare the samples for subsequent thermal processing, where flavonols facilitate myosin unfolding and enhance intermolecular aggregation, thereby improving gel strength. This process also transforms free water within the gel to bound water, significantly increasing the water-holding capacity. Both macroscopic and microscopic rheological analyses suggest that flavonols, particularly isorhamnetin, notably enhance the elasticity and viscosity of myosin during the gelation process. Further, microstructural examinations employing techniques such as small-angle X-ray scattering and micro-computed tomography demonstrate that samples treated with flavonols develop denser and more uniform gel networks. Analytical studies reveal that flavonols, especially methylated derivatives like isorhamnetin, effectively promote gelation through strengthening hydrophobic interactions, disulfide bonds, and ionic interactions among myosin molecules. This research underscores the potential of flavonols as natural additives to enhance the quality and functional properties of surimi products, thereby elevating the processing standards of aquatic products.
Keywords: Flavonols; Gel strength; Microstructure; Myosin; Rheological properties; Sliver carp.
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