Baculovirus Expressing Tumor Growth Factor-β1 (TGFβ1) Nanoshuttle Augments Therapeutic Effects for Vascular Wound Healing: Design and In Vitro Analysis

Paromita Islam; Ahmed Abosalha; Sabrina Schaly; Jacqueline L Boyajian; Madison Santos; Stephanie Makhlouf; Editha Renesteen; Amal Kassab; Cedrique Shum-Tim; Dominique Shum-Tim; Satya Prakash

doi:10.1021/acsptsci.4c00509

Baculovirus Expressing Tumor Growth Factor-β1 (TGFβ1) Nanoshuttle Augments Therapeutic Effects for Vascular Wound Healing: Design and In Vitro Analysis

ACS Pharmacol Transl Sci. 2024 Oct 25;7(11):3419-3428. doi: 10.1021/acsptsci.4c00509. eCollection 2024 Nov 8.

Authors

Affiliations

¹ Biomedical Technology and Cell Therapy Research Laboratory, Department of Biomedical Engineering, Faculty of Medicine and Health Sciences, McGill University, Montreal, Quebec H3A 2B4, Canada.
² Pharmaceutical Technology Department, Faculty of Pharmacy, Tanta University, Tanta Al-Geish St., the Medical Campus, Tanta 31527, Egypt.
³ Mila-Quebec AI Institute, McGill University, 6666 Saint-Urbain Street, Montreal, Quebec H2S 3H1, Canada.
⁴ Division of Cardiac Surgery, Royal Victoria Hospital, McGill University Health Centre, Faculty of Medicine and Health Sciences, McGill University, Montreal, Quebec H3G 2M1, Canada.

PMID: 39539270
PMCID: PMC11555499 (available on 2025-10-25)
DOI: 10.1021/acsptsci.4c00509

Abstract

One of the major challenges in vascular tissue regeneration is effective wound healing that can be resolved by an innovative targeted nanoshuttle that delivers growth factors to blood vessels. This study investigates the production and efficacy of transforming growth factor-β1 (TGFβ1) gene delivery using poly(lactic-co-glycolic acid) (PLGA) baculovirus (BV) nanoshuttles (NSs). They exhibited an encapsulation efficiency of 86.23% ± 0.65% and a negative zeta potential of -29.57 ± 1.27 mV. In vitro studies in human umbilical vein endothelial cells (HUVECs) revealed that a 12 h incubation period optimized virus transduction. The safety and superior intracellular uptake of NSs and BVs in HUVECs were observed. The NSs carrying 100 and 400 MOI exhibited the highest cell proliferation rates in HUVECs. These sustained-release NSs significantly improved vascular cell migration and wound closure compared to free TGFβ1 carrying BV and can be a groundbreaking find in regenerative medicine, cardiovascular diseases, and chronic ulcer conditions.