Hsa_circRNA_000166 accelerates breast cancer progression via the regulation of the miR-326/ELK1 and miR-330-5p/ELK1 axes

Ann Med. 2024 Dec;56(1):2424515. doi: 10.1080/07853890.2024.2424515. Epub 2024 Nov 12.

Abstract

Purposes: To probe the expression, clinical significance, roles, and molecular mechanisms of circRNA_000166 in breast cancer (BC).

Methods: Clinical tissue samples were gathered from 84 BC patients who underwent surgery at the Affiliated Hospital of Chengde Medical College. Clinical data were obtained from medical records and postoperative follow-up. Expression levels of circRNA_000166, miR-326, miR-330-5p, and ELK1 mRNA in BC tissues and cells were measured by qRT-PCR, and ELK1 protein levels were assessed by WB. Pearson's correlation analysis evaluated the interrelationships between these RNAs in clinical samples. Luciferase reporter assays verified the interactions between miR-326/miR-330-5p and circRNA_000166, as well as between miR-326/miR-330-5p and ELK1. Cell proliferation, migration, and apoptosis were examined using CCK-8, colony formation, transwell, and flow cytometry assays, respectively.

Results: CircRNA_000166 was highly expressed in BC tissues and inversely correlated with miR-326/miR-330-5p levels but positively with ELK1 mRNA levels. ELK1 mRNA also inversely associated with miR-326/miR-330-5p levels in BC tissues. Importantly, our findings demonstrated that circRNA_000166 targets miR-326 and miR-330-5p, while ELK1 is the target of miR-326 and miR-330-5p in BC cells. CircRNA_000166 levels positively correlated with tumour size, TNM stage, histological grade, and lymph node metastasis, and negatively associated with postoperative progression-free survival (PFS) and overall survival (OS) in BC patients. CircRNA_000166 was also highly expressed in BC cells, and knockdown of circRNA_000166 reduced proliferation and migration, and increased apoptosis via miR-326/ELK1 and miR-330-5p/ELK1 pathways in vitro.

Conclusion: CircRNA_000166 enhances BC progression through miR-326/ELK1 and miR-330-5p/ELK1 pathways and shows potential as a biomarker for BC diagnosis and treatment.

Keywords: Breast cancer; biomarker; circRNA_000166/miR-326/ELK1 axis; circRNA_000166/miR-330-5p/ELK1 axis; malignant biological behaviours.

MeSH terms

  • Adult
  • Apoptosis / genetics
  • Breast Neoplasms* / genetics
  • Breast Neoplasms* / metabolism
  • Breast Neoplasms* / pathology
  • Cell Line, Tumor
  • Cell Movement / genetics
  • Cell Proliferation* / genetics
  • Disease Progression*
  • Female
  • Gene Expression Regulation, Neoplastic*
  • Humans
  • MicroRNAs* / genetics
  • MicroRNAs* / metabolism
  • Middle Aged
  • RNA, Circular* / genetics
  • RNA, Circular* / metabolism
  • ets-Domain Protein Elk-1* / genetics
  • ets-Domain Protein Elk-1* / metabolism

Substances

  • MicroRNAs
  • ets-Domain Protein Elk-1
  • RNA, Circular
  • MIRN326 microRNA, human
  • ELK1 protein, human
  • MIRN330 microRNA, human

Grants and funding

No external funding sources supported this study.