Sublethal effects of microplastic and oil co-exposure on biological rates and lipid profiles of keystone Arctic copepods

R Almeda; R Rodriguez-Torres; S Rist; M Winding; S Jonasdottir; T Gissel Nielsen

doi:10.1016/j.envpol.2024.125286

Sublethal effects of microplastic and oil co-exposure on biological rates and lipid profiles of keystone Arctic copepods

Environ Pollut. 2024 Nov 9;363(Pt 2):125286. doi: 10.1016/j.envpol.2024.125286. Online ahead of print.

Authors

R Almeda¹, R Rodriguez-Torres², S Rist³, M Winding⁴, S Jonasdottir³, T Gissel Nielsen³

Affiliations

¹ EOMAR, ECOAQUA, University of Las Palmas de Gran Gran Canaria, Spain; DTU AQUA, Technical University of Denmark, Denmark. Electronic address: rodrigo.almeda@ulpgc.es.
² DTU AQUA, Technical University of Denmark, Denmark; Laboratoire d'Océanographie de Villefranche sur mer (LOV), Sorbonne Université, France.
³ DTU AQUA, Technical University of Denmark, Denmark.
⁴ Greenland Climate Research Centre, Greenland Institute of Natural Resources, Greenland.

PMID: 39522637
DOI: 10.1016/j.envpol.2024.125286

Abstract

Microplastics (MPs) and petroleum hydrocarbons are contaminants of emerging concern in the Arctic, but little is known about their co-exposure effects. In this study, we present the first assessment of the sublethal impacts resulting from combined exposure to microplastics and oil in three key Arctic copepod species. Specifically, we investigated the effects of a 5-day exposure to oil alone (1 μL L^-1) and in combination with MPs (polyethylene microspheres, 20 μm, 20 MP mL^-1) and dispersant (Corexit 9500, 0.05 μL L^-1) on the biological functions and lipid profiles of the planktonic copepods Metridia longa, Calanus finmarchicus, and Calanus glacialis. Exposure to oil alone caused a significant reduction (34-58%) in fecal pellet production, but neither microplastics nor dispersant increased the negative effect of oil on fecal pellet production. C. glacialis and C. finmarchicus exposed to the studied pollutants for 5 days produced eggs with delayed hatching and lower hatching success. The highest hatching inhibition (50%) was observed in eggs of C. glacialis exposed to oil plus MPs and dispersant for five days. This indicates that maternal transfer of oil components to eggs negatively affects embryonic development and hatching. Lipid content and fatty acids profiles varied among the studied copepod species but were not affected by the tested pollutants after five days of exposure. By microscopical observation of fecal pellets, ingestion of small oil droplets and MPs was confirmed in all species, but the estimated ingestion of MPs was low (<25 MPs cop^-1 d^-1, <0.2% of total offered MPs) suggesting avoidance of MP consumption in copepods. Our results indicate that virgin MPs did not increase the toxicity of oil to the studied Arctic copepods under co-exposure conditions, and dispersants can slightly increase certain adverse effects of oil (hatching). However, environmentally relevant concentrations of oil alone can negatively impact Arctic keystone copepods and potentially the biological carbon pump. These findings emphasize the need to reduce petrogenic pollution and the risk of oil spills in the sensitive Arctic ecosystem.

Keywords: Arctic copepods; Crude oil; Dispersant; Lipid profiles; Microplastics; Sublethal effect.