Physically crosslinked chitosan/αβ-glycerophosphate hydrogels enhanced by surface-modified cyclodextrin: An efficient strategy for controlled drug release

Jianan Tong; Quang Nhat Quynh Vo; Xichan He; Hongyu Liu; Huiyun Zhou; Chan Hee Park

doi:10.1016/j.ijbiomac.2024.137163

Physically crosslinked chitosan/αβ-glycerophosphate hydrogels enhanced by surface-modified cyclodextrin: An efficient strategy for controlled drug release

Int J Biol Macromol. 2024 Nov 5;283(Pt 1):137163. doi: 10.1016/j.ijbiomac.2024.137163. Online ahead of print.

Authors

Jianan Tong¹, Quang Nhat Quynh Vo¹, Xichan He², Hongyu Liu³, Huiyun Zhou⁴, Chan Hee Park⁵

Affiliations

¹ Department of Bionanotechnology and Bioconvergence Engineering, Graduate School, Jeonbuk National University, Jeonju 54896, Republic of Korea.
² School of Materials Science and Engineering, Luoyang Institute of Science and Technology, Luoyang 471023, China.
³ School of Chemistry & Chemical Engineering, Henan University of Science and Technology, Luoyang 471023, China. Electronic address: liuhy2003@ustc.edu.
⁴ School of Chemistry & Chemical Engineering, Henan University of Science and Technology, Luoyang 471023, China. Electronic address: hyzhou@haust.edu.cn.
⁵ Department of Bionanotechnology and Bioconvergence Engineering, Graduate School, Jeonbuk National University, Jeonju 54896, Republic of Korea; Department of Bionanosystem Engineering, Graduate School, Jeonbuk National University, Jeonju 54896, Republic of Korea; Department of Mechanical Design Engineering, Graduate School, Jeonbuk National University, Jeonju 54896, Republic of Korea; Advanced Mechanical Components Design & Research Center, Jeonbuk National University, Jeonju 54896, Republic of Korea. Electronic address: biochan@jbnu.ac.kr.

PMID: 39510462
DOI: 10.1016/j.ijbiomac.2024.137163

Abstract

This study reports physically crosslinked chitosan/αβ-glycerophosphate (CS/GP) hydrogels containing surface-modified cyclodextrin for efficient controlled drug release. Highly water-soluble β-cyclodextrin-grafted L-serine (CD-g-Ser) compounds were synthesized, and employed as an effective carrier of berberine hydrochloride (Ber) for CS/GP hydrogels. Various characterizations, including gelation time determination, scanning electron microscopy, and viscosity measurement, indicated that the introduction of CD-g-Ser led to increased crosslinking degree, improved temperature sensitivity, and shortened sol-gel phase transition time of the hydrogel. Meanwhile, the sustained release ability for Ber was achieved due to the hydrophobic association between cyclodextrin and Ber. It was observed that within 4 h, the hydrogel containing CD-g-Ser released 40 % of Ber, while the CS/GP hydrogel without CD-g-Ser released 65 % of Ber. Furthermore, in vitro bacteriostasis experiments confirmed the drug-loaded hydrogel had an excellent antibacterial effect against E. coli and S. aureus (diameter of the inhibition zone up to (16.4 and 34.7) mm, respectively), low hemolysis rate (<2 %), and high cell viability (>90 %). The findings indicate that the physical crosslinked CS hydrogel can be used as a new drug delivery system, and its excellent antibacterial effect makes it a potential wound dressing candidate.

Keywords: Chitosan hydrogel; Controlled drug release; Physically crosslink.