Overexpression of blaSHV-12 caused by tandem amplification contributed to ceftazidime/avibactam resistance in hypervirulent and carbapenem-resistant Klebsiella pneumoniae

Chao Liu; Juan Yi; Ping Yang; Chunjing Du; Fan Jiang; Ming Lu; Pengcheng Du; Ning Shen

doi:10.1080/22221751.2024.2426481

Overexpression of bla_SHV-12 caused by tandem amplification contributed to ceftazidime/avibactam resistance in hypervirulent and carbapenem-resistant Klebsiella pneumoniae

Emerg Microbes Infect. 2024 Nov 5:2426481. doi: 10.1080/22221751.2024.2426481. Online ahead of print.

Authors

Chao Liu^{1

2}, Juan Yi³, Ping Yang³, Chunjing Du^{2

4}, Fan Jiang⁴, Ming Lu^{1

2}, Pengcheng Du⁵, Ning Shen^{1

2

3

4}

Affiliations

¹ Department of Infectious Disease, Peking University Third Hospital, Beijing, China.
² Center of Infectious Disease, Peking University Third Hospital, Beijing, China.
³ Institute of Medical Technology, Peking University Health Science Center, Beijing, China.
⁴ Department of Pulmonary and Critical Care Medicine, Peking University Third Hospital, Beijing, China.
⁵ Qitan Technology Ltd., Chengdu, China.

PMID: 39498854
DOI: 10.1080/22221751.2024.2426481

Abstract

AbstractWe identified a novel ceftazidime/avibactam (CAZ/AVI) resistance mechanism in endemic sequence type 11 hypervirulent and carbapenem-resistant Klebsiella pneumoniae isolated from a patient who had not been exposed CAZ/AVI. Overexpression of bla_SHV-12 caused by tandem gene amplification contributed to CAZ/AVI resistance instead of the carriage of bla_KPC-2. Enhanced genomic surveillance is essential to identify emerging variants.

Keywords: blaSHV-12; ceftazidime/avibactam resistance; gene amplification; hypervirulent klebsiella pneumoniae; sequence type 11.