Single gold nanowire-nanoparticles conjugated system: Fabrication and sensing application

Qingshan Dai; Yanyan Zhu; Xianzhun Luo; Yongxin Li

doi:10.1016/j.talanta.2024.127134

Single gold nanowire-nanoparticles conjugated system: Fabrication and sensing application

Talanta. 2024 Oct 31:283:127134. doi: 10.1016/j.talanta.2024.127134. Online ahead of print.

Authors

Qingshan Dai¹, Yanyan Zhu¹, Xianzhun Luo¹, Yongxin Li²

Affiliations

¹ Anhui Province Key Laboratory of Biomedical Materials and Chemical Measurement, Key Laboratory of Functional Molecular Solids, Ministry of Education, College of Chemistry and Materials Science, Anhui Normal University, Wuhu, 241000, PR China.
² Anhui Province Key Laboratory of Biomedical Materials and Chemical Measurement, Key Laboratory of Functional Molecular Solids, Ministry of Education, College of Chemistry and Materials Science, Anhui Normal University, Wuhu, 241000, PR China. Electronic address: yongli@mail.ahnu.edu.cn.

PMID: 39488156
DOI: 10.1016/j.talanta.2024.127134

Abstract

Single-entity-based sensing platform has many advantages for real-time assays, such as single-molecule analysis, or targets detection in confined environment. In this contribution, a new single Au nanowire (NW) - Au@Pt/Au nanoparticles (Au@Pt/Au NPs) conjugated system was established and used for the detection of thrombin by using surface-enhanced Raman spectroscopy (SERS) technique. This method was mainly based on electrostatic attraction between the capture (thrombin aptamer) and probe molecules (crystal violet, CV) on the surface of Au NW - Au@Pt/Au NPs conjugation, reducing the adsorption of CV molecules on conjugation surface, and resulting the decrease of SERS signals. The addition of thrombin could bind with thrombin aptamer due to specific interaction, leading to the decrease of electrostatic effect from thrombin aptamer for CV diffusion to conjugation surface, and the SERS signals could be recovered. This thrombin sensing method showed high sensitivity and selectivity, and the linear range for thrombin assay was 0.01 nM-100 nM with a detection limit of 1.35 pM. This single Au NW - Au@Pt/Au NPs conjugation has more SERS hotspots and small overall size, which offers unparalleled advantages over other sensing system and can be applicable for real time analysis, especially at single molecule/cell level.

Keywords: Au@Pt/Au nanoparticles; Single Au nanowire; Surface-enhanced Raman scattering; Thrombin.