Biocontrol activity of Kluyveromyces marxianus YG-4 against Penicillium expansum LPH9 on apples

Mengge Ning; Qi Guo; Peng Guo; Yuanyuan Cui; Kai Wang; Gengan Du; Zhouli Wang; Yahong Yuan; Tianli Yue

doi:10.1016/j.ijfoodmicro.2024.110943

Biocontrol activity of Kluyveromyces marxianus YG-4 against Penicillium expansum LPH9 on apples

Int J Food Microbiol. 2024 Oct 22:427:110943. doi: 10.1016/j.ijfoodmicro.2024.110943. Online ahead of print.

Authors

Mengge Ning¹, Qi Guo², Peng Guo¹, Yuanyuan Cui¹, Kai Wang¹, Gengan Du¹, Zhouli Wang¹, Yahong Yuan³, Tianli Yue⁴

Affiliations

¹ College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China.
² College of Food Science and Technology, Henan Agr Univ, Zhengzhou 450002, China.
³ College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China; College of Food Science and Technology, Northwest University, Xi'an 710069, China. Electronic address: yyh324@126.com.
⁴ College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China; College of Food Science and Technology, Northwest University, Xi'an 710069, China. Electronic address: yuetl305@nwsuaf.edu.cn.

PMID: 39454321
DOI: 10.1016/j.ijfoodmicro.2024.110943

Abstract

Penicillium expansum (P. expansum), a widespread fungal pathogen, causes serious economic loss and public health concerns. The aim of this research is to investigate the antifungal effect of Kluyveromyces marxianus YG-4 (K. marxianus YG-4) against P. expansum and possible mechanism. The results showed that competition for nutrients and space, as well as the release of volatile organic compounds (VOCs), are the antifungal mechanisms. Citronellol may be the antifungal component of K. marxianus YG-4 VOCs based on GC-MS analysis. Further experiments had shown that citronellol inhibited the growth of P. expansum LPH9 by damaging the cell structure, disrupting the redox system, reducing antioxidant enzyme activity, and causing oxidative damage. K. marxianus YG-4, K. marxianus YG-4 VOCs and citronellol can effectively inhibit the spore germination of P. expansum on apples. The above results indicated that K. marxianus YG-4 had strong biocontrol activity and can be used as an excellent candidate strain for fruit preservation.

Keywords: Antifungal mechanism; Apple; Citronellol; Kluyveromyces marxianus; Penicillium expansum; Volatile organic compounds.