Unraveling the Core Components and Critical Targets of Houttuynia cordata Thunb. in Treating Non-small Cell Lung Cancer through Network Pharmacology and Multi-omics Analysis

Jinyan Yang; Yang Li; Yan Zhang; Ling Xu; Jiahui Wang; Feng Xing; Xinqiang Song

doi:10.2174/0113816128330427241017110325

Unraveling the Core Components and Critical Targets of Houttuynia cordata Thunb. in Treating Non-small Cell Lung Cancer through Network Pharmacology and Multi-omics Analysis

Curr Pharm Des. 2024 Oct 21. doi: 10.2174/0113816128330427241017110325. Online ahead of print.

Authors

Jinyan Yang¹, Yang Li², Yan Zhang¹, Ling Xu¹, Jiahui Wang³, Feng Xing¹, Xinqiang Song^{1

4}

Affiliations

¹ College of Life Science, Xinyang Normal University, Xinyang 464000, China.
² Department of Ultrasound, Xinyang Central Hospital, Xinyang 464000, China.
³ College of International Education, Xinyang Normal University, Xinyang 464000, China.
⁴ Medical College, Xinyang Normal University, Xinyang 464000, China.

PMID: 39440769
DOI: 10.2174/0113816128330427241017110325

Abstract

Objective: This study aimed to preliminary explore the molecular mechanisms of Houttuynia cordata Thunb. (H. cordata; Saururaceae) in treating non-small cell lung cancer (NSCLC), with the goal of screening drug potential targets for clinical drug development.

Methods: This study employed a multi-omics and multi-source data integration approach to identify potential therapeutic targets of H. cordata against NSCLC from the TCMSP database, GEO database, BioGPS database, Metascape database, and others. Meanwhile, target localization was performed, and its possible mechanisms of action were predicted. Furthermore, dynamics simulations and molecular docking were used for verification. Multiomics analysis was used to confirm the selected key genes' efficacy in treating NSCLC.

Results: A total of 31 potential therapeutic targets, 8 key genes, and 5 core components of H. cordata against NSCLC were screened out. These potential therapeutic targets played a therapeutic role mainly by regulating lipid and atherosclerosis, the TNF signaling pathway, the IL-17 signaling pathway, and others. Molecular docking indicated a stable combination between MMP9 and quercetin. Finally, through multi-omics analysis, it was found that the expression of some key genes was closely related not only to the progression and prognosis of NSCLC but also to the level of immune infiltration.

Conclusion: Through comprehensive network pharmacology and multi-omics analysis, this study predicts that the core components of H. cordata play a role in treating NSCLC by regulating lipid and atherosclerosis, as well as the TNF signaling pathway. Among them, the anti-NSCLC activity of isoramanone is reported for the first time.

Keywords: Houttuynia cordata Thunb.; drug targets.; molecular docking; molecular dynamics simulation; network pharmacology; non-small cell lung cancer.