Taxonomic intestinal microbiota differences in Lewy body spectrum disease and cohabitant controls

Levi M Teigen; Stuart J McCarter; Zachary Ziegert; Christopher Staley; Kiera M Grant; Vinod K Gupta; Xiaowei Zhao; Erik K St Louis; Kejal Kantarci; Val J Lowe; Leah K Forsberg; Rodolfo Savica; Vijay K Ramanan; David T Jones; Ronald C Petersen; Jaeyun Sung; Alexander Khoruts; Bradley F Boeve; Owen A Ross

doi:10.1016/j.parkreldis.2024.107176

Taxonomic intestinal microbiota differences in Lewy body spectrum disease and cohabitant controls

Parkinsonism Relat Disord. 2024 Oct 16:129:107176. doi: 10.1016/j.parkreldis.2024.107176. Online ahead of print.

Authors

Levi M Teigen¹, Stuart J McCarter², Zachary Ziegert³, Christopher Staley³, Kiera M Grant⁴, Vinod K Gupta⁵, Xiaowei Zhao⁶, Erik K St Louis², Kejal Kantarci⁷, Val J Lowe⁷, Leah K Forsberg⁸, Rodolfo Savica⁸, Vijay K Ramanan⁸, David T Jones⁸, Ronald C Petersen⁸, Jaeyun Sung⁹, Alexander Khoruts¹⁰, Bradley F Boeve², Owen A Ross¹¹

Affiliations

¹ Department of Food Science and Nutrition, University of Minnesota, St. Paul, MN, 55108, USA. Electronic address: teige027@umn.edu.
² Department of Neurology, Mayo Clinic, Rochester, MN, 55905, USA; Center for Sleep Medicine, Mayo Clinic, Rochester, MN, 55905, USA.
³ Department of Surgery, School of Medicine, University of Minnesota, Minneapolis, MN, 55455, USA.
⁴ Department of Biomedical Statistics and Informatics, Mayo Clinic, Rochester, MN, 55905, USA.
⁵ Microbiomics Program, Center for Individualized Medicine, Mayo Clinic, Rochester, MN, 55905, USA.
⁶ Bioinformatics and Computational Biology Program, University of Minnesota, Rochester, MN, 55904, USA.
⁷ Department of Radiology, Mayo Clinic, Rochester, MN, 55905, USA.
⁸ Department of Neurology, Mayo Clinic, Rochester, MN, 55905, USA.
⁹ Microbiomics Program, Center for Individualized Medicine, Mayo Clinic, Rochester, MN, 55905, USA; Bioinformatics and Computational Biology Program, University of Minnesota, Rochester, MN, 55904, USA.
¹⁰ Division of Gastroenterology, Department of Medicine, University of Minnesota, Minneapolis, MN, 55455, USA.
¹¹ Department of Neuroscience, Mayo Clinic, Jacksonville, FL, 32224, USA.

PMID: 39426329
DOI: 10.1016/j.parkreldis.2024.107176

Abstract

Background: Microbial dysbiosis has been reported to contribute to development of neurodegenerative diseases, however, there is a need to identify causative/prognostic indicators.

Objectives: To comparatively analyze gut microbiome composition in symptomatic LBD (dementia/mild cognitive impairment), iRBD, and cohabiting controls without LBD or iRBD.

Methods: 16S rRNA amplicon sequencing was performed in 38 cases (27 LBD, 11 iRBD) and 39 cohabitant controls. 19 non-cohabitant healthy controls (HCs) were also included to contrast differences between cohabitant cases and controls.

Results: Microbiome composition of cohabitant controls and LBD and iRBD cases were strikingly similar. No differences were observed between LBD, and iRBD only showed reduced Bacteroides, compared with cohabitant controls. There were several taxonomic differences in gut microbiome composition between non-cohabitant HCs and cases.

Conclusions: Minimal microbiome differences were observed between iRBD or LBD cases and cohabitant controls. These findings underscore the importance of using cohabiting controls in future gut microbiome studies.