Alterations in the gut microbiota in patients with long-term follow-up after pancreaticoduodenectomy and their association with postoperative fatty liver: A pilot study

Yuichiro Uchida; Tadashi Fujii; Hideaki Takahashi; Kazunori Nakaoka; Kohei Funasaka; Eizaburo Ohno; Yoshiki Hirooka; Takeshi Takahara; Koichi Suda; Takumi Tochio

doi:10.1016/j.pan.2024.10.005

Alterations in the gut microbiota in patients with long-term follow-up after pancreaticoduodenectomy and their association with postoperative fatty liver: A pilot study

Pancreatology. 2024 Oct 3:S1424-3903(24)00755-5. doi: 10.1016/j.pan.2024.10.005. Online ahead of print.

Authors

Affiliations

¹ Department of Surgery, Fujita Health University, Japan.
² Department of Medical Research on Prebiotics and Probiotics, Fujita Health University, Japan; Department of Gastroenterology and Hepatology, Fujita Health University, Japan; BIOSIS Lab. Co., Ltd., Aichi, Japan. Electronic address: tadashi.fujii@fujita-hu.ac.jp.
³ Department of Medical Research on Prebiotics and Probiotics, Fujita Health University, Japan; Department of Gastroenterology and Hepatology, Fujita Health University, Japan; BIOSIS Lab. Co., Ltd., Aichi, Japan.
⁴ Department of Gastroenterology and Hepatology, Fujita Health University, Japan.

PMID: 39419749
DOI: 10.1016/j.pan.2024.10.005

Abstract

Background/purpose: Gut microbiota status after pancreaticoduodenectomy (PD) is unclear, and postoperative fatty liver is an important complication after PD. This study evaluated the relationship between postoperative fatty liver and gut microbiota after PD.

Methods: Fecal samples were collected from patients who had undergone PD and remained stable after 6 months of follow-up. A comprehensive bacterial analysis using 16S rRNA gene amplicon sequencing was performed. The results were compared with those of 85 healthy volunteers. The association between perioperative factors, gut microbiota, and development of fatty liver was investigated.

Results: Twenty-four patients after PD, including 10 in the fatty liver (FL) group and 14 in the normal liver (NL) group were investigated. The β-diversity of the gut microbiota was significantly different between the healthy volunteers and patients after PD, with more Escherichia coli and Streptococcus gallolyticus and less Bifidobacterium catenulatum and Faecalibacterium prausnitzii in the patients with PD. Lactobacillus gasseri was significantly less abundant in the FL group than in the healthy volunteers, although this change was not observed in the NL group.

Conclusions: The gut microbiota of patients after PD was in dysbiosis at postoperative ≥6 months. Development of fatty liver might be associated with significant differences in gut microbiota.

Keywords: Dysbiosis; Fatty liver; Gut microbiota; Pancreaticoduodenectomy; Prebiotics.