In this study, the effects of chitosan-coating on maintaining the integrity and stability of the membrane, structural, and morphological changes, and the release of loaded peptides inside nanoliposomes during various in vitro release, thermal, freeze-thaw, shear, and dehydration (spray-drying) tensions were evaluated. Among different peptidic fractions (100, 30, and 10 kDa), the Arthrospira derived PF-30 kDa showed a higher nutritional and biological value. PF-30kDa was loaded successfully (EE ~ 90 %) inside nanoliposomes (NLs) and its stabilization was done with chitosan coating (0.1-0.8 %). Nanochitosomes (NCs-0.4 %) had more structural stability (size, EE, and biological activity) at different temperatures, freeze-thaw tension, and digestive system. The placement of peptides in the vesicle structure was confirmed by FTIR analysis. Also, the changes in the morphological states, agglomeration, or destruction of the liposome membrane (SEM, AFM, and TEM) were evaluated before and after the tensions. Membrane coating led to the transformation of freeze-dried liposomes (FD-NLs) from thin, porous, and fragile layers to thick plates, rough and resistant structures (FD-NCs). These characteristics led to maintaining physical stability, homogeneity, zeta potential, and EE of nanoparticles (freeze and spray-dried) after reconstitution. The results of this study will effectively contribute to the production of solidified delivery systems with long-term durability, bioavailability, and biological activity of loaded nutrients and drugs.
Keywords: Controlled release; Membrane stability; Peptides fraction; Prochitosomes; Spray-drying.
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