MicroRNA-124a promoted the differentiation of bone marrow mesenchymal stem cells into neurons through Notch signal pathway

Eur J Med Res. 2024 Sep 28;29(1):472. doi: 10.1186/s40001-024-02061-6.

Abstract

This study investigated the possible mechanisms of microRNA-124a on the differentiation of bone marrow mesenchymal stem cells (BMSCs) and its underlying mechanism. β-Thiol ethanol induced Notch1 mRNA expression, microRNA-124a inhibitor reduced the effects of β-thiol ethanol on Notch1 mRNA expression in BMSCs. Baicalin induced Hes1 mRNA expression, and microRNA-124a inhibitor reduced the effects of baicalin on Hes1 mRNA expression in BMSCs. Si-Notch1 suppressed Hes1 mRNA expression in BMSCs. Baicalin increased the effects of Notch1 on Hes1 mRNA expression in BMSCs. Si-Notch1 increased cell growth of BMSCs. Baicalin reduced the effects of si-Notch1 on cell growth and the differentiation of BMSCs. We demonstrated that microRNA-124a promoted the differentiation of BMSCs into neurons through Notch/Hes1 signal pathway.

Keywords: BMSCs; Hes1; MicroRNA-124a; Notch.

MeSH terms

  • Animals
  • Bone Marrow Cells / drug effects
  • Bone Marrow Cells / metabolism
  • Cell Differentiation* / drug effects
  • Cell Proliferation / drug effects
  • Cells, Cultured
  • Flavonoids / pharmacology
  • Mesenchymal Stem Cells* / drug effects
  • Mesenchymal Stem Cells* / metabolism
  • MicroRNAs* / genetics
  • MicroRNAs* / metabolism
  • Neurons* / cytology
  • Neurons* / metabolism
  • Rats
  • Rats, Sprague-Dawley
  • Receptor, Notch1* / genetics
  • Receptor, Notch1* / metabolism
  • Receptors, Notch / genetics
  • Receptors, Notch / metabolism
  • Signal Transduction* / drug effects
  • Transcription Factor HES-1* / genetics
  • Transcription Factor HES-1* / metabolism

Substances

  • MicroRNAs
  • Receptor, Notch1
  • Transcription Factor HES-1
  • baicalin
  • MIRN124 microRNA, rat
  • Flavonoids
  • Hes1 protein, rat
  • Receptors, Notch