Investigation of genotoxicity induced by intermediate frequency magnetic field combined with ionizing radiation: In vitro study on human fibroblast cells

Mutat Res Genet Toxicol Environ Mutagen. 2024 Oct:899:503817. doi: 10.1016/j.mrgentox.2024.503817. Epub 2024 Aug 10.

Abstract

These days, exposure to electromagnetic fields has become omnipresent in modern society. Not only the extremely-low frequency and radiofrequency, but also intermediate frequency (IF) magnetic field (MF) might be absorbed in the human body resulting in an ever-growing concern about their possible health effects. Devices, such as induction cooktops, chargers, compact fluorescent lamps, touchscreens and electric vehicles emit a wide range of intermediate frequency fields. We investigated the effects of 22 kHz or 250 kHz intermediate frequency magnetic field exposure on the human skin cells. We also examined the adaptive response phenomenon; whether IF MF exposure could possibly reduce the harmful genotoxic effects of ionizing radiation. To get answers to these questions, in vitro studies were carried out on fibroblast cells to investigate the effects on oxidative stress, DNA damage and micronucleus formation. We found a decreased micronucleus formation due to the 22 kHz IF MF exposure and significantly increased oxidative stress in fibroblast cells, which were exposed only to 250 kHz IF MF. We were unable to detect the protective or co-genotoxic effects of intermediate frequency magnetic field exposure combined with ionizing radiation, thus we found no evidence for the adaptive response phenomena.

Keywords: Adaptive response; Genotoxicity; In vitro; Intermediate frequency; Oxidative stress; Skin.

MeSH terms

  • Cell Line
  • Cells, Cultured
  • DNA Damage* / radiation effects
  • Electromagnetic Fields / adverse effects
  • Fibroblasts* / metabolism
  • Fibroblasts* / radiation effects
  • Humans
  • Magnetic Fields* / adverse effects
  • Micronucleus Tests*
  • Oxidative Stress* / radiation effects
  • Radiation, Ionizing*