IL-22 resolves MASLD via enterocyte STAT3 restoration of diet-perturbed intestinal homeostasis

Cell Metab. 2024 Oct 1;36(10):2341-2354.e6. doi: 10.1016/j.cmet.2024.08.012. Epub 2024 Sep 23.

Abstract

The exponential rise in metabolic dysfunction-associated steatotic liver disease (MASLD) parallels the ever-increasing consumption of energy-dense diets, underscoring the need for effective MASLD-resolving drugs. MASLD pathogenesis is linked to obesity, diabetes, "gut-liver axis" alterations, and defective interleukin-22 (IL-22) signaling. Although barrier-protective IL-22 blunts diet-induced metabolic alterations, inhibits lipid intake, and reverses microbial dysbiosis, obesogenic diets rapidly suppress its production by small intestine-localized innate lymphocytes. This results in STAT3 inhibition in intestinal epithelial cells (IECs) and expansion of the absorptive enterocyte compartment. These MASLD-sustaining aberrations were reversed by administration of recombinant IL-22, which resolved hepatosteatosis, inflammation, fibrosis, and insulin resistance. Exogenous IL-22 exerted its therapeutic effects through its IEC receptor, rather than hepatocytes, activating STAT3 and inhibiting WNT-β-catenin signaling to shrink the absorptive enterocyte compartment. By reversing diet-reinforced macronutrient absorption, the main source of liver lipids, IL-22 signaling restoration represents a potentially effective interception of dietary obesity and MASLD.

Keywords: IL-22; MASLD; STAT3; WNT-β-catenin; hepatosteatosis; lipid absorption.

MeSH terms

  • Animals
  • Diet
  • Diet, High-Fat / adverse effects
  • Enterocytes* / metabolism
  • Fatty Liver / metabolism
  • Fatty Liver / pathology
  • Homeostasis
  • Humans
  • Interleukin-22* / metabolism
  • Interleukins / metabolism
  • Intestinal Mucosa / metabolism
  • Intestines / drug effects
  • Intestines / pathology
  • Male
  • Mice
  • Mice, Inbred C57BL
  • STAT3 Transcription Factor* / metabolism

Substances

  • Interleukin-22
  • Interleukins
  • Stat3 protein, mouse
  • STAT3 Transcription Factor