An Inhalable Nanoshield for Effective Prevention of Influenza Virus Infections

ACS Nano. 2024 Oct 8;18(40):27327-27339. doi: 10.1021/acsnano.4c04631. Epub 2024 Sep 24.

Abstract

Influenza virus (IV) infection currently poses a serious and continuing threat to the global public health. Developing effective prevention strategies is important to defend against infection and spread of IV. Here, we developed a triple-protective nanoshield against IV infection in the lungs, formed by self-assembling DSPE-PEG amphiphilic polymers encapsulating the flu-preventive antiviral drug Arbidol internally. The preventive effect of the nanoshield against virus infection includes increasing the viscosity in the surrounding environment to physically defend against viral entry, forming a hydrated layer to block the interaction between viruses and cells, and inhibiting virus replication. Our finding suggested that a single inhalation of the nanoshield provides effective protection against IV infection for at least 8 h. Thus, this nanoshield may be a potential pandemic protection agent against IV, especially in viral environments, where no prophylactic or therapeutic measures are available.

Keywords: amphiphilic polymers; antiviral defense; influenza virus; nanoshield; virus infection.

MeSH terms

  • Administration, Inhalation
  • Animals
  • Antiviral Agents* / administration & dosage
  • Antiviral Agents* / chemistry
  • Antiviral Agents* / pharmacology
  • Female
  • Humans
  • Influenza, Human / prevention & control
  • Madin Darby Canine Kidney Cells
  • Mice
  • Mice, Inbred BALB C
  • Nanoparticles / chemistry
  • Orthomyxoviridae Infections / prevention & control
  • Orthomyxoviridae Infections / virology
  • Polyethylene Glycols / chemistry

Substances

  • Antiviral Agents
  • Polyethylene Glycols