High-loading ficin@AuNPs on polymer-UiO-66 surface with enhanced peroxidase-mimetic catalytic activity for colourimetric detection of dopamine

Yutong Liu; Lin Tian; Zhenwen Zhao; Wei Liu; Li Qi

doi:10.1007/s00604-024-06689-3

High-loading ficin@AuNPs on polymer-UiO-66 surface with enhanced peroxidase-mimetic catalytic activity for colourimetric detection of dopamine

Mikrochim Acta. 2024 Sep 23;191(10):616. doi: 10.1007/s00604-024-06689-3.

Authors

Yutong Liu^{1

2}, Lin Tian^{1

2}, Zhenwen Zhao^{1

3}, Wei Liu⁴, Li Qi^{5

6}

Affiliations

¹ Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Beijing National Laboratory for Molecular Sciences, Chinese Academy of Sciences, Beijing, 100190, P. R. of China.
² School of Pharmacy, Xinxiang Medical University, Xinxiang, 453003, P. R. China.
³ School of Chemical Sciences, University of Chinese Academy of Sciences, Beijing, 100049, P. R. China.
⁴ School of Pharmacy, Xinxiang Medical University, Xinxiang, 453003, P. R. China. liuwei@xxmu.edu.cn.
⁵ Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Beijing National Laboratory for Molecular Sciences, Chinese Academy of Sciences, Beijing, 100190, P. R. of China. qili@iccas.ac.cn.
⁶ School of Chemical Sciences, University of Chinese Academy of Sciences, Beijing, 100049, P. R. China. qili@iccas.ac.cn.

PMID: 39313731
DOI: 10.1007/s00604-024-06689-3

Abstract

Recently, MOFs@AuNPs composites-based catalysts via anchoring of AuNPs onto metal-organic-frameworks (MOFs) have attracted great attention. However, the influence of the AuNPs loading amounts on the catalytic activity of MOFs@AuNPs composites remains largely unexplored. Here, ficin (Fic) protected AuNPs (Fic@AuNPs) anchored onto the surface of UiO-66-NH₂ (UiO) modified with poly(2-vinyl-4,4-dimethyl-2-oxazolidine) (PV) were designed and constructed. The UiOPVFic@AuNPs composites with longer PV chains leading to high-loading Fic@AuNPs exhibited intense peroxidase (POD)-mimetic activity in 3,3'5,5'-tetramethylbenzidine (TMB) oxidation. Further, following the colour-fading, dopamine (DA) was sensitively and selectively monitored in the composites-TMB-H₂O₂ system. The portable smartphone sensing platform-based colourimetric method had good linearity ranging from 3.34 to 36.7 μM (R² = 0.995), with a limit of detection of 0.3 μM. This protocol explores high-loading AuNPs on polymer-MOFs composites, providing deep insights into understanding catalytic activity improvements of polymer-MOFs@AuNPs catalysts and revealing their application potential in real biological samples analysis.

Keywords: Fic@AuNPs; Metal organic framework; Peroxidase-mimetic activity; Poly (2-vinyl-4,4-dimethyl-2-oxazolidine); Surface loading.

Grants and funding

22274159/National Natural Science Foundation of China