miR-351-5p regulation of CPEB3 affecting aluminium-induced learning and memory impairment in SD rats

Jingjing Ji; Yirong Xu; Yanni Wang; Guangheng Zhang; Xiaoai Tian; Yeping Zhang; Jiaxuan Ren

doi:10.1016/j.envpol.2024.124973

miR-351-5p regulation of CPEB3 affecting aluminium-induced learning and memory impairment in SD rats

Environ Pollut. 2024 Sep 20:124973. doi: 10.1016/j.envpol.2024.124973. Online ahead of print.

Authors

Jingjing Ji¹, Yirong Xu², Yanni Wang³, Guangheng Zhang⁴, Xiaoai Tian⁵, Yeping Zhang⁴, Jiaxuan Ren⁴

Affiliations

¹ Shanxi Medical University Fenyang College, China; Pathology Department , Shanxi Fenyang Hospital, China.
² Shanxi Medical University Fenyang College, China; Pathology Department , Shanxi Fenyang Hospital, China. Electronic address: yirongxu@163.com.
³ School of public health , Shanxi Medical University, China.
⁴ Shanxi Medical University Fenyang College, China.
⁵ Pathology Department , Shanxi Fenyang Hospital, China.

PMID: 39307336
DOI: 10.1016/j.envpol.2024.124973

Abstract

Aluminium exposure has been found to impair learning and memory abilities; however, the underlying molecular mechanisms remain unclear. In this study we conducted a double luciferase reporter assay to determine whether miR-351-5p regulates cytoplasmic polyadenylation element binding protein (CPEB) 3 mRNA. To this end, we overexpressed and inhibited miR-351-5p via stereotaxic microinjections of adeno-associated virus (AAV) into the hippocampus of Sprague Dawley rats in a sub-chronic aluminium exposure model to examine learning and memory ability using Morris water maze. Ultrastructural electron microscopy and Golgi staining were used to examine morphological changes in hippocampal neurons. In addition, we examined the levels of synaptic plasticity-related proteins (PRPs) and CPEB3 to determine the involvement of the miR-351-5P/CPEB3/PRPs pathway in aluminium neurotoxicity. Sub-chronic aluminium exposure reduced the spatial learning and memory ability of rats. Overexpression of AAV-miR-351-5P in the hippocampus aggravated the impairment of spatial learning and memory abilities of aluminium-treated rats, whereas inhibition of AAV-miR-351-5p expression alleviated it. Western blotting suggested that sub-chronic aluminium exposure increased miR-351-5p levels and reduced the expression of CPEB3 and PRPs in the hippocampus. Treatment with an AAV-miR-351-5p inhibitor partially recovered CPEB3 and PRPs. Double luciferase reporter assay results showed that CPEB3 was a direct target of miR-351-5p, while electron microscopy suggested that aluminium could damage mitochondria and synapses in the CA1 of the hippocampus. Golgi staining results indicated that aluminium could reduce the number of dendritic spines in hippocampal neurons. Inhibition of miR-351-5p restored the synaptic structure and growth of dendritic spines in the hippocampus. The involvement of the miR-351-5P/CPEB3/RPPs pathway in aluminium neurotoxicity was confirmed. Our findings suggest that inhibition of miR-351-5p can alleviate learning and memory impairments by increasing CPEB3 and PRPs.

Keywords: CPEB3; SD rats; aluminium; hippocampus; miRNA-351-5P; neurotoxicity.