Effect of H2O2/ascorbic acid degradation and gradient ethanol precipitation on the physicochemical properties and biological activities of pectin polysaccharides from Satsuma Mandarin

Hekai Fan; Ruoxuan Li; Yanbing Zhang; Xiaoyun Xu; Siyi Pan; Fengxia Liu

doi:10.1016/j.ijbiomac.2024.135843

Effect of H₂O₂/ascorbic acid degradation and gradient ethanol precipitation on the physicochemical properties and biological activities of pectin polysaccharides from Satsuma Mandarin

Int J Biol Macromol. 2024 Sep 20;280(Pt 3):135843. doi: 10.1016/j.ijbiomac.2024.135843. Online ahead of print.

Authors

Hekai Fan¹, Ruoxuan Li¹, Yanbing Zhang¹, Xiaoyun Xu¹, Siyi Pan¹, Fengxia Liu²

Affiliations

¹ College of Food Science and Technology, Huazhong agricultural university, Wuhan, Hubei, PR China; Hubei Key Laboratory of Fruit & Vegetable Processing & Quality Control, Huazhong Agricultural University, Wuhan, Hubei, PR China.
² College of Food Science and Technology, Huazhong agricultural university, Wuhan, Hubei, PR China; Hubei Key Laboratory of Fruit & Vegetable Processing & Quality Control, Huazhong Agricultural University, Wuhan, Hubei, PR China. Electronic address: liufxia@mail.hzau.edu.cn.

PMID: 39306161
DOI: 10.1016/j.ijbiomac.2024.135843

Abstract

In this work, three degraded polysaccharides (DMPP-40, DMPP-60, DMPP-80) were successfully obtained by H₂O₂/ascorbic acid degradation and gradient ethanol precipitation from Satsuma mandarin peel pectin (MPP), and their physicochemical properties, antioxidant and prebiotic activities were investigated. The molecular weight of MPP, DMPP-40, DMPP-60, DMPP-80 were determined to be 336.83 ± 10.57, 18.93 ± 0.54, 26.07 ± 0.83 and 8.71 ± 0.27 kDa, respectively. The ethanol concentration significantly affected the physicochemical properties of DMPPs. DMPP-60 showed the highest yield (69.07 %) and uronic acid content (64.85 %), DMPP-80 showed the lowest molecular weight (8.71 kDa), and the composition and proportion of monosaccharides of DMPPs were significantly different. Fourier transform infrared spectroscopy (FT-IR) and nuclear magnetic resonance spectroscopy (¹H NMR) confirmed that DMPPs exhibited similar functional groups, while X-ray diffraction (XRD) indicated that DMPP-40 possessed some crystallographic sequences. Scanning electron microscopy (SEM) images directly verified the fragmented structure and reduced surface area of DMPPs. Besides, the H₂O₂/ascorbic acid treatment could obviously reduce the apparent viscosity and thermal stability of MPP. Meanwhile, the results of bioactivity assay showed that DMPPs possessed better antioxidant activity and probiotics pro-proliferative effects compared with MPP. DMPP-80 could significantly inhibit lipopolysaccharides (LPS)-stimulated production of inflammatory factors (including nitric oxide (NO), interleukin (IL)-6, tumor necrosis factor (TNF)-α and interleukin (IL)-1β) in RAW264.7 cells. Results suggest that the H₂O₂/ascorbic acid combined with gradient ethanol precipitation has potential applications in degradation and separation of MPP to improve its biological activities.

Keywords: Biological activity; Gradient ethanol precipitation; H(2)O(2)/ascorbic acid degradation; Pectic polysaccharide; Structural characteristic.