The Epstein-Barr virus small capsid protein BFRF3 disrupts the NF-кB signaling pathway by inhibiting p65 activity

FASEB J. 2024 Sep 30;38(18):e23820. doi: 10.1096/fj.202400952R.

Abstract

Epstein-Barr virus (EBV), a common gamma herpesvirus, establishes a life-long latent infection in the host to defend against innate immune recognition, which is closely related to a variety of malignant tumors, but its specific mechanism is unclear. BFRF3, an EBV-encoded small capsid protein, is mainly involved in the assembly of the viral capsid structure and the maintenance of its stability. Here, we showed that BFRF3 can inhibit TNF-α-mediated NF-кB promoter activation. Moreover, BFRF3 downregulates NF-кB-mediated promoter activation and transcription of inflammatory cytokines, including IL-6 and IL-8. Dual-luciferase reporter assay demonstrated that BFRF3 restrains NF-кB promoter activity at or below the p65 level, and coimmunoprecipitation analysis revealed that BFRF3 not only interacts with p65 but also binds to its critical truncated Rel homology domain (RHD) and transcriptional activation domain (TAD). However, BFRF3 does not affect the dimerization of p65-p50, but overexpression of BFRF3 reduces the nuclear accumulation of p65, and the phosphorylation of p65 (Ser536) is repressed during BFRF3 transfection and EBV lytic infection, which promotes the proliferation of EBV. Overall, our study suggested that BFRF3 may play a crucial role in antiviral immunity to defend against EBV infection by inhibiting NF-κB activity.

Keywords: BFRF3; NF‐кB; phosphorylation of p65 in EBV.

MeSH terms

  • Capsid Proteins* / genetics
  • Capsid Proteins* / metabolism
  • Epstein-Barr Virus Infections / immunology
  • Epstein-Barr Virus Infections / metabolism
  • Epstein-Barr Virus Infections / virology
  • HEK293 Cells
  • Herpesvirus 4, Human* / immunology
  • Herpesvirus 4, Human* / metabolism
  • Herpesvirus 4, Human* / physiology
  • Humans
  • NF-kappa B* / metabolism
  • Promoter Regions, Genetic
  • Signal Transduction*
  • Transcription Factor RelA* / metabolism
  • Tumor Necrosis Factor-alpha / metabolism

Substances

  • Capsid Proteins
  • Transcription Factor RelA
  • NF-kappa B
  • RELA protein, human
  • Tumor Necrosis Factor-alpha

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