EV71 infection alters the lipid composition of human rhabdomyosarcoma (RD) cells-derived extracellular vesicles

Lingxiang Mao; Qing Gao; Yuxuan Shen; Chenxuan Bao; Huayuan Xiang; Qiaoqiao Chen; Qianqian Gao; Feng Huang; Wenyuan He; Jianjun Wang

doi:10.3389/fmicb.2024.1430052

EV71 infection alters the lipid composition of human rhabdomyosarcoma (RD) cells-derived extracellular vesicles

Front Microbiol. 2024 Sep 5:15:1430052. doi: 10.3389/fmicb.2024.1430052. eCollection 2024.

Authors

Lingxiang Mao^#¹, Qing Gao^#¹, Yuxuan Shen², Chenxuan Bao¹, Huayuan Xiang¹, Qiaoqiao Chen^{1

3}, Qianqian Gao¹, Feng Huang¹, Wenyuan He¹, Jianjun Wang¹

Affiliations

¹ Department of Laboratory Medicine, Affiliated Kunshan Hospital of Jiangsu University, Kunshan, Jiangsu, China.
² Medical College, Yangzhou University, Yangzhou, Jiangsu, China.
³ Department of Laboratory Medicine, Affiliated People's Hospital of Jiangsu University, Zhenjiang, Jiangsu, China.

^# Contributed equally.

Abstract

Previous studies demonstrated that EV71-infected cells secrete extracellular vesicles (EVs), facilitating the transfer of viral components to recipient cells and thereby promoting virus spread. Considering lipid signaling plays a crucial role in EVs-mediated cell-to-cell communication, we compared the lipid profile of EVs secreted from uninfected and EV71-infected cells (EVs-Mock and EVs-EV71) using the human rhabdomyosarcoma (RD) cell model. These two groups of EVs were purified by using size exclusion chromatography (SEC), respectively, and evaluated by transmission electron microscopy (TEM), nanoparticle tracking technology (NTA), and Western blotting (WB). In-depth lipidomic analysis of EVs identified 1705 lipid molecules belonging to 43 lipid classes. The data showed a significant increase in the lipid content of EVs after EV71 infection. Meanwhile, we deeply analyzed the changes in lipids and screened for lipid molecules with significant differences compared EVs-EV71 with EVs-Mock EVs. Altogether, we report the alterations in the lipid profile of EVs derived from RD-cells after EV71 infection, which may affect the function of the EVs in the recipient cells.

Keywords: EV71; SEC; extracellular vesicles; lipid; lipidomics.

Grants and funding

The authors declare that financial support was received for the research, authorship, and/or publication of this article. This research was funded by the National Natural Science Foundation of China (32070182), the Key Project of Jiangsu Commission of Health (ZD2021049), Suzhou municipal science and technology development plan (SKYD2023002), Suzhou municipal key discipline construction project (SZXK202124), and the talent research project of Suzhou health talent plan (GSWS2023005).