Isolation, structural elucidation and biosynthetic pathway of bioactive prenyl quinone compounds from Panus lecomtei based on untargeted metabolomics combined with molecular networking

Si-Xian Wang; Bao-Song Chen; Zi-Juan Zhang; Shi-Rong Zhu; Xiao-Ling Wang; Gao-Qiang Liu

doi:10.1016/j.foodchem.2024.141275

Isolation, structural elucidation and biosynthetic pathway of bioactive prenyl quinone compounds from Panus lecomtei based on untargeted metabolomics combined with molecular networking

Food Chem. 2024 Sep 12;463(Pt 2):141275. doi: 10.1016/j.foodchem.2024.141275. Online ahead of print.

Authors

Si-Xian Wang¹, Bao-Song Chen², Zi-Juan Zhang¹, Shi-Rong Zhu¹, Xiao-Ling Wang³, Gao-Qiang Liu⁴

Affiliations

¹ Hunan Provincial Key Laboratory of Forestry Biotechnology & International Cooperation Base of Science and Technology Innovation on Forest Resource Biotechnology, Central South University of Forestry & Technology, Changsha 410004, China.
² State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China; Savaid Medicine School, University of Chinese Academy of Sciences, Beijing 100049, China.
³ Hunan Provincial Key Laboratory of Forestry Biotechnology & International Cooperation Base of Science and Technology Innovation on Forest Resource Biotechnology, Central South University of Forestry & Technology, Changsha 410004, China. Electronic address: wxlcsedu@163.com.
⁴ Hunan Provincial Key Laboratory of Forestry Biotechnology & International Cooperation Base of Science and Technology Innovation on Forest Resource Biotechnology, Central South University of Forestry & Technology, Changsha 410004, China. Electronic address: gaoliuedu@csuft.edu.cn.

PMID: 39298853
DOI: 10.1016/j.foodchem.2024.141275

Abstract

Panus lecomtei is a relatively unfamiliar and undeveloped mushroom. This study generated ethyl acetate extracts of P. lecomtei intracellular (I), extracellular (E) and total fermentation broth (T). Both E and T extracts demonstrated antioxidant and antibacterial activities at 100 to 200 μg/mL. The composition differences of metabolites of these extracts were further studied based on comparative metabolomics by LS/MS and molecular network analysis. The results revealed that there were over 2000 significantly distinct metabolites among the three extracts, with abundant prenyl quinone compounds. Furthermore, the molecular network clarified the conversion relationship of P. lecomtei metabolites. Seven known prenyl quinone derivatives (1-7) were isolated from the E extract. Among them, compound 3 displayed excellent antioxidant activity and modest antibacterial activity. Compound 5 was discovered in fungi for the first time. Finally, a potential biosynthetic route for prenyl quinone in P. lecomtei was suggested.

Keywords: Biosynthetic pathway; Edible and medicinal fungi; Isolation; Metabolomics; Panus lecomtei; Prenyl quinone.