Christensenella minuta protects and restores intestinal barrier in a colitis mouse model by regulating inflammation

NPJ Biofilms Microbiomes. 2024 Sep 19;10(1):88. doi: 10.1038/s41522-024-00540-6.

Abstract

Christensenella minuta DSM 22607 has recently been suggested as a potential microbiome-based therapy for inflammatory bowel disease (IBD) because it displays strong anti-inflammatory effects both in vitro and in vivo. Here, we aimed to decipher the mechanism(s) underlying the DSM 22607-mediated beneficial effects on the host in a mouse model of chemically induced acute colitis. We observed that C. minuta plays a key role in the preservation of the epithelial barrier and the management of DNBS-induced inflammation by inhibiting interleukin (IL)-33 and Tumor necrosis factor receptor superfamily member 8 (Tnfrsf8) gene expression. We also showed that DSM 22607 abundance was positively correlated with Akkermansia sp. and Dubosiella sp. and modulated microbial metabolites in the cecum. These results offer new insights into the biological and molecular mechanisms underlying the beneficial effects of C. minuta DSM 22607 by protecting the intestinal barrier integrity and regulating inflammation.

MeSH terms

  • Animals
  • Cecum / microbiology
  • Clostridiales
  • Colitis* / chemically induced
  • Dinitrofluorobenzene / analogs & derivatives
  • Disease Models, Animal*
  • Gastrointestinal Microbiome / drug effects
  • Inflammation
  • Intestinal Mucosa* / drug effects
  • Intestinal Mucosa* / metabolism
  • Intestinal Mucosa* / microbiology
  • Mice
  • Mice, Inbred C57BL

Substances

  • 2,4-dinitrofluorobenzene sulfonic acid
  • Dinitrofluorobenzene