Isolation and genetic characterization of novel bovine parechoviruses from Japanese black cattle

Arch Virol. 2024 Sep 16;169(10):200. doi: 10.1007/s00705-024-06120-5.

Abstract

Novel bovine parechoviruses (Bo ParVs) were isolated from the feces of Japanese black cattle. Phylogenetic analysis revealed that the novel Bo ParVs formed an independent cluster, exhibiting 72.2-75.6% nucleotide sequence identity to previous Bo ParVs, suggesting that they represent a new genotype. Bo ParVs, including the novel Bo ParVs, shared sequence similarity with each other in the 3' untranslated region (3'UTR) and exhibited low sequence similarity (<38.9% identity) to other parechoviruses. However, a secondary structure prediction of the 3'UTR revealed that the Bo ParVs shared conserved motifs in domain 2 with parechovirus B and E, suggesting some evolutionary constrains in this region.

MeSH terms

  • 3' Untranslated Regions / genetics
  • Animals
  • Cattle
  • Cattle Diseases* / virology
  • Feces* / virology
  • Genotype
  • Japan
  • Nucleic Acid Conformation
  • Parechovirus* / classification
  • Parechovirus* / genetics
  • Parechovirus* / isolation & purification
  • Phylogeny*
  • Picornaviridae Infections* / veterinary
  • Picornaviridae Infections* / virology
  • RNA, Viral / genetics

Substances

  • 3' Untranslated Regions
  • RNA, Viral

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