Two new species of Fusarium in the F. incarnatum-equiseti species complex from Oryza sativa in Iran

Antonie Van Leeuwenhoek. 2024 Sep 16;118(1):5. doi: 10.1007/s10482-024-02019-y.

Abstract

Identification of Fusarium species associated with diseases symptoms in plants is an important step toward understanding the ecology of plant-fungus associations. In this study, four Fusarium isolates were obtained from root rot of Oryza sativa L. in Izeh (southwest of Iran) and identified based on phylogenetic analyses combined with morphology. Phylogenetic analyses based on combined translation elongation factor 1-α, calmodulin, RNA polymerase II second largest subunit, and Beta-tubulin (tub2) sequence data delimited two new species, namely F. khuzestanicum and F. oryzicola spp. nov., from previously known species of Fusarium within F. incarnatum-equiseti species complex (FIESC). Morphologically, F. khuzestanicum produces the macroconidia with distinctly notched to foot-shaped basal cells, while basal cells in the macroconidia of F. oryzicola are more extended and distinctly elongated foot shape. Furthermore, these two new species are distinguished by the size of their sporodochial phialides and macroconidia. The results of the present show that the FIESC species complex represent more cryptic species.

Keywords: Macroconidia; Morphology; Multi-locus phylogenetic analyses; Two new species.

MeSH terms

  • Calmodulin / genetics
  • DNA, Fungal / genetics
  • Fusarium* / classification
  • Fusarium* / genetics
  • Fusarium* / isolation & purification
  • Iran
  • Oryza* / microbiology
  • Peptide Elongation Factor 1 / genetics
  • Phylogeny*
  • Plant Diseases* / microbiology
  • Plant Roots / microbiology
  • RNA Polymerase II / genetics
  • Tubulin / genetics

Substances

  • Tubulin
  • Calmodulin
  • RNA Polymerase II
  • DNA, Fungal
  • Peptide Elongation Factor 1