Highly sensitive detection of carbendazim in agricultural products using colorimetric and photothermal lateral flow immunoassay based on plasmonic gold nanostars

Lei Liu; Tian Zhang; Qi Wu; Longyingzi Xie; Qiyang Zhao; Yaohai Zhang; Yongliang Cui; Chengqiu Wang; Yue He

doi:10.1016/j.talanta.2024.126891

Highly sensitive detection of carbendazim in agricultural products using colorimetric and photothermal lateral flow immunoassay based on plasmonic gold nanostars

Talanta. 2025 Jan 1:281:126891. doi: 10.1016/j.talanta.2024.126891. Epub 2024 Sep 14.

Authors

Lei Liu¹, Tian Zhang¹, Qi Wu¹, Longyingzi Xie¹, Qiyang Zhao¹, Yaohai Zhang¹, Yongliang Cui¹, Chengqiu Wang², Yue He³

Affiliations

¹ Key Laboratory of Quality and Safety Control of Citrus Fruits, Ministry of Agriculture and Rural Affairs, Southwest University, Chongqing, 400712, PR China; Laboratory of Quality & Safety Risk Assessment for Citrus Products (Chongqing), Ministry of Agriculture and Rural Affairs, Citrus Research Institute, Southwest University, Chongqing, 400712, PR China; National Citrus Engineering Research Center, Chongqing, 400712, PR China.
² Key Laboratory of Quality and Safety Control of Citrus Fruits, Ministry of Agriculture and Rural Affairs, Southwest University, Chongqing, 400712, PR China; Laboratory of Quality & Safety Risk Assessment for Citrus Products (Chongqing), Ministry of Agriculture and Rural Affairs, Citrus Research Institute, Southwest University, Chongqing, 400712, PR China; National Citrus Engineering Research Center, Chongqing, 400712, PR China. Electronic address: wangchengqiu@cric.cn.
³ Key Laboratory of Quality and Safety Control of Citrus Fruits, Ministry of Agriculture and Rural Affairs, Southwest University, Chongqing, 400712, PR China; Laboratory of Quality & Safety Risk Assessment for Citrus Products (Chongqing), Ministry of Agriculture and Rural Affairs, Citrus Research Institute, Southwest University, Chongqing, 400712, PR China; National Citrus Engineering Research Center, Chongqing, 400712, PR China. Electronic address: yuehe@cric.cn.

PMID: 39277934
DOI: 10.1016/j.talanta.2024.126891

Abstract

The wide use and high toxicity of carbendazim (CBD) in agriculture pose unprecedented demands for convenient, sensitive, and cost-effective on-site monitoring. Herein, we propose a novel colorimetric and photothermal dual-mode lateral flow immunoassay (LFIA) based on plasmonic gold nanostars (AuNSs) for CBD detection in agricultural products. The AuNSs were synthesized via a rapid seed-mediated growth method (with growth time of ∼5 s). A stable immunoprobe was formed by adsorbing CBD antibodies onto AuNSs. This immunoprobe exhibited high conversion efficiency and sensitivity in photothermal detection with a low limit of detection (LOD) of 0.28 ng mL^-1. The LOD of the colorimetric mode was higher (0.48 ng mL^-1). The results of CBD detection in various agricultural products aligned well with ultra-performance liquid chromatography tandem mass spectrometry. Overall, our LFIA shows excellent sensitivity, specificity, reproducibility, and rapidness in CBD detection, and thus is a highly potential on-site platform in resource-limited environments.

Keywords: Food safety; Gold nanostars; Lateral flow immunoassay; Pesticide; Plasmonic nanomaterials.

MeSH terms

Benzimidazoles* / analysis
Benzimidazoles* / chemistry
Carbamates* / analysis
Colorimetry* / methods
Gold* / chemistry
Immunoassay / methods
Limit of Detection
Metal Nanoparticles* / chemistry

Substances

Gold
Carbamates
carbendazim
Benzimidazoles