Dysmetabolism of Peripheral Blood Monocytes in Type 1 Diabetes Mellitus

V V Ivanov; E E Buyko; A A Ufandeev; K V Nevskaya; Ya S Slavkina; E V Udut; T V Saprina; V V Udut

doi:10.1007/s10517-024-06201-0

Dysmetabolism of Peripheral Blood Monocytes in Type 1 Diabetes Mellitus

Bull Exp Biol Med. 2024 Sep 12. doi: 10.1007/s10517-024-06201-0. Online ahead of print.

Authors

V V Ivanov¹, E E Buyko², A A Ufandeev², K V Nevskaya², Ya S Slavkina², E V Udut², T V Saprina², V V Udut³

Affiliations

¹ Siberian State Medical University, Ministry of Health of Russia, Tomsk, Russia. ivanovvv1953@gmail.com.
² Siberian State Medical University, Ministry of Health of Russia, Tomsk, Russia.
³ Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk National Research Medical Center, Russian Academy of Sciences, Tomsk, Russia.

PMID: 39264558
DOI: 10.1007/s10517-024-06201-0

Abstract

The level of ROS (fluorescent probe 2',7'-dichlorodihydrofluorescein diacetate) and lipid content (fluorescent lipophilic dye Nile Red) in the peripheral blood monocyte fraction from patients with type 1 diabetes mellitus and healthy volunteers were assessed by flow cytofluorimetry. The number of CD36⁺ monocytes was assessed using specific antibodies. In patients with type 1 diabetes mellitus, the levels of ROS and intracellular lipids in monocytes and the number of cells expressing CD36 fatty acid translocase were elevated. These results indicate metabolic changes in the peripheral blood cells of patients with carbohydrate metabolism disorders and can be considered as possible prognostic markers for the development of type 1 diabetes mellitus complications.

Keywords: CD36; free fatty acids; monocytes; reactive oxygen species; type 1 diabetes mellitus.