Trends in developing one-pot CRISPR diagnostics strategies

Lin Chen; Menglu Hu; Xiaoming Zhou

doi:10.1016/j.tibtech.2024.07.007

Trends in developing one-pot CRISPR diagnostics strategies

Trends Biotechnol. 2024 Aug 1:S0167-7799(24)00185-9. doi: 10.1016/j.tibtech.2024.07.007. Online ahead of print.

Authors

Lin Chen¹, Menglu Hu², Xiaoming Zhou³

Affiliations

¹ School of Life sciences, South China Normal University, Guangzhou 510631, P. R. China.
² School of Life sciences, South China Normal University, Guangzhou 510631, P. R. China. Electronic address: humenglu2018@163.com.
³ School of Life sciences, South China Normal University, Guangzhou 510631, P. R. China; MOE Key laboratory of Laser Life Science & Guangdong Provincial Key Laboratory of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China. Electronic address: zhouxm@scnu.edu.cn.

PMID: 39095257
DOI: 10.1016/j.tibtech.2024.07.007

Abstract

The integration of nucleic acid amplification (NAA) with the CRISPR detection system has led to significant advancements and opportunities for development in molecular diagnostics. Nevertheless, the incompatibility between CRISPR cleavage and NAA has significantly impeded the commercialization of this technology. Currently, several one-pot detection strategies based on CRISPR systems have been devised to address concerns regarding aerosol contamination risk and operational complexity associated with step-by-step detection as well as the sensitivity limitation of conventional one-pot methods. In this review, we provide a comprehensive introduction and outlook of the various solutions of the one-pot CRISPR assay for practitioners who are committed to developing better CRISPR nucleic acid detection technologies to promote the progress of molecular diagnostics.

Keywords: CRISPR–Cas system; nucleic acid amplification; nucleic acid detection; one-pot assay.

Publication types

Review