Protein Kinase A Inhibitor Attenuates the Antinociceptive Effect of NMDA-Receptor Channel Antagonists in the Capsaicin Test in Mice

Bull Exp Biol Med. 2024 Jun;177(2):231-234. doi: 10.1007/s10517-024-06162-4. Epub 2024 Aug 2.

Abstract

Acute nociceptive pain in mice caused by subcutaneous (intraplantar) injection of TRPV1 ion channel agonist capsaicin (1.6 μg/mouse) and the effects of protein kinase A inhibitor H-89 (0.05 mg/mouse, intraplantar injection) and NMDA receptor channel antagonists MK-801 (7.5 and 15 μg/mouse, topical application) and hemantane (0.5 mg/mouse, topical application) on the pain were assessed. MK-801 and hemantane were found to reduce the duration of the pain response. H-89 did not significantly affect the pain in animals, but preliminary administration of this drug abolished the antinociceptive effect of MK-801 (7.5 μg/mouse) and weakens the effect of hemantane (0.5 mg/mouse).

Keywords: NMDA receptor; TRPV1 ion channel; mice; nociceptive pain; protein kinase A.

MeSH terms

  • Analgesics* / pharmacology
  • Animals
  • Capsaicin* / pharmacology
  • Cyclic AMP-Dependent Protein Kinases / antagonists & inhibitors
  • Cyclic AMP-Dependent Protein Kinases / metabolism
  • Dizocilpine Maleate* / pharmacology
  • Male
  • Mice
  • Nociceptive Pain / chemically induced
  • Nociceptive Pain / drug therapy
  • Pain Measurement / drug effects
  • Pain Measurement / methods
  • Protein Kinase Inhibitors / pharmacology
  • Receptors, N-Methyl-D-Aspartate* / antagonists & inhibitors
  • TRPV Cation Channels / antagonists & inhibitors
  • TRPV Cation Channels / metabolism

Substances

  • Capsaicin
  • Receptors, N-Methyl-D-Aspartate
  • Dizocilpine Maleate
  • Analgesics
  • TRPV Cation Channels
  • Cyclic AMP-Dependent Protein Kinases
  • Protein Kinase Inhibitors
  • TRPV1 protein, mouse