Trastuzumab Deruxtecan in Advanced Solid Tumors With Human Epidermal Growth Factor Receptor 2 Amplification Identified by Plasma Cell-Free DNA Testing: A Multicenter, Single-Arm, Phase II Basket Trial

J Clin Oncol. 2024 Nov 10;42(32):3817-3825. doi: 10.1200/JCO.23.02626. Epub 2024 Aug 1.

Abstract

Purpose: HERALD/EPOC1806 was conducted as a multicenter phase II trial assessing trastuzumab deruxtecan (T-DXd) therapy for patients with human epidermal growth factor receptor 2 (HER2)-amplified progressive stage solid tumors detected by cell-free DNA (cfDNA) testing.

Patients and methods: Patients exhibited advanced solid tumors with HER2 amplification that was identified via next-generation sequencing of cfDNA testing, without the requirement for immunohistochemical HER2 testing. The studied group was administered T-DXd at 5.4 mg/kg once every 3 weeks until onset of disease progression or intolerable toxicity.

Results: Overall, 4,734 patients underwent cfDNA testing from December 2019 to January 2022, and 252 demonstrated HER2 amplification. Finally, the study included 62 patients with 16 cancer types with a median baseline plasma HER2 copy number (CN) of 8.55 (range, 2.4-73.9). Confirmed overall response rate (ORR) by investigator assessment was 56.5% (95% CI, 43.3 to 69.0), thus showing a value beyond the 5% threshold. Responses were evaluated for 13 cancer types, including KRAS-mutant colorectal (1/3), PIK3CA-mutant endometrial (5/6), and tissue HER2-negative gastric (1/2) cancers. Plasma HER2 CN above versus below the baseline median value did not differ for impact response; however, clearance of HER2 amplification in cfDNA on cycle 2 day 1 had higher response values compared with persistence. Median progression-free survival and response duration were 7.0 (95% CI, 4.9 to 9.7) and 8.8 (95% CI, 5.8 to 11.2) months, respectively, with the majority of complications being mild to moderate. Interstitial lung diseases were identified in 16 (26%) patients, including 14 patients with grade 1 disease, one patient with grade 2 disease, and one patient with grade 3 disease.

Conclusion: T-DXd treatment demonstrated high ORR with durable response in patients with advanced HER2-amplified solid tumors determined with cfDNA testing.

Publication types

  • Clinical Trial, Phase II
  • Multicenter Study

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Antineoplastic Agents, Immunological / therapeutic use
  • Biomarkers, Tumor / blood
  • Biomarkers, Tumor / genetics
  • Camptothecin / analogs & derivatives
  • Camptothecin / therapeutic use
  • Cell-Free Nucleic Acids* / blood
  • Circulating Tumor DNA / blood
  • Circulating Tumor DNA / genetics
  • Female
  • Gene Amplification
  • Humans
  • Immunoconjugates / administration & dosage
  • Immunoconjugates / therapeutic use
  • Male
  • Middle Aged
  • Neoplasms* / blood
  • Neoplasms* / drug therapy
  • Neoplasms* / genetics
  • Receptor, ErbB-2* / analysis
  • Receptor, ErbB-2* / genetics
  • Trastuzumab* / therapeutic use

Substances

  • Receptor, ErbB-2
  • ERBB2 protein, human
  • Trastuzumab
  • trastuzumab deruxtecan
  • Cell-Free Nucleic Acids
  • Camptothecin
  • Antineoplastic Agents, Immunological
  • Immunoconjugates
  • Circulating Tumor DNA
  • Biomarkers, Tumor