Botanical formulation HX110B ameliorates PPE-induced emphysema in mice via regulation of PPAR/RXR signaling pathway

PLoS One. 2024 Jul 25;19(7):e0305911. doi: 10.1371/journal.pone.0305911. eCollection 2024.

Abstract

Chronic obstructive pulmonary disease (COPD), an inflammatory lung disease, causes approximately 3 million deaths each year; however, its pathological mechanisms are not fully understood. In this study, we examined whether HX110B, a mixture of Taraxacum officinale, Dioscorea batatas, and Schizonepeta tenuifolia extracts, could suppress porcine pancreatic elastase (PPE)-induced emphysema in mice and its mechanism of action. The therapeutic efficacy of HX110B was tested using a PPE-induced emphysema mouse model and human bronchial epithelial cell line BEAS-2B. In vivo data showed that the alveolar wall and air space expansion damaged by PPE were improved by HX110B administration. HX110B also effectively suppresses the expression levels of pro-inflammatory mediators including IL-6, IL-1β, MIP-2, and iNOS, while stimulating the expression of lung protective factors such as IL-10, CC16, SP-D, and sRAGE. Moreover, HX110B improved the impaired OXPHOS subunit gene expression. In vitro analysis revealed that HX110B exerted its effects by activating the PPAR-RXR signaling pathways. Overall, our data demonstrated that HX110B could be a promising therapeutic option for COPD treatment.

MeSH terms

  • Animals
  • Cell Line
  • Disease Models, Animal
  • Humans
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Pancreatic Elastase* / metabolism
  • Peroxisome Proliferator-Activated Receptors / metabolism
  • Plant Extracts* / pharmacology
  • Pulmonary Disease, Chronic Obstructive / drug therapy
  • Pulmonary Disease, Chronic Obstructive / metabolism
  • Pulmonary Disease, Chronic Obstructive / pathology
  • Pulmonary Emphysema / chemically induced
  • Pulmonary Emphysema / drug therapy
  • Pulmonary Emphysema / metabolism
  • Pulmonary Emphysema / pathology
  • Signal Transduction* / drug effects
  • Swine

Substances

  • Pancreatic Elastase
  • Plant Extracts
  • Peroxisome Proliferator-Activated Receptors

Grants and funding

This work was financially supported by Helixmith Co., Ltd.