Exosome Therapy and Photobiomodulation Therapy Regulate mi-RNA 21, 155 Expressions, Nucleus Acetylation and Glutathione in a Polycystic Ovary Oocyte: An In Vitro Study

Samira Sahraeian; Hojjat Allah Abbaszadeh; Robabeh Taheripanah; Mohammad Amin Edalatmanesh; Somayeh Keshavarzi; Alaleh Ghazifard

doi:10.34172/jlms.2024.10

Exosome Therapy and Photobiomodulation Therapy Regulate mi-RNA 21, 155 Expressions, Nucleus Acetylation and Glutathione in a Polycystic Ovary Oocyte: An In Vitro Study

J Lasers Med Sci. 2024 Apr 30:15:e10. doi: 10.34172/jlms.2024.10. eCollection 2024.

Authors

Samira Sahraeian¹, Hojjat Allah Abbaszadeh^{2

3}, Robabeh Taheripanah⁴, Mohammad Amin Edalatmanesh¹, Somayeh Keshavarzi⁵, Alaleh Ghazifard⁵

Affiliations

¹ Department of Biology, College of Sciences, Shiraz Branch, Islamic Azad University, Shiraz, Iran.
² Laser Applications in Medical Sciences Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
³ Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
⁴ Department of Obstetrics and Gynecology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
⁵ Infertility Clinic, Erfan Niayesh Hospital, Iran University of Medical Sciences, Tehran, Iran.

Abstract

Introduction: Polycystic ovary syndrome (PCOS) is a complex condition that can have various symptoms and complications, one of which is infertility. Dysregulation of miRNA has been associated with the pathogenesis of numerous illnesses such as PCOS. In this study, we evaluated the effect of photobiomodulation therapy (PBMT) and exosome therapy (EXO) on the regulation of miRNA and nucleus acetylation in a PCOS oocyte. Methods: In this research, 36 oocytes divided into three groups: control, EXO, and PBM (Wavelength of 640 nm). Subsequently, in-vitro maturation (IVM) was evaluated. Real-time PCR was used to evaluate miRNA-21,16,19,24,30,106,155 and GAPDH. Afterward, oocyte glutathione (GSH) and nucleus acetylation were measured by H4K12. Results: The expression of the miR-16, miRNA-19, miRNA-24, miRNA-106 and miRNA-155 genes in the EXO and PBMT groups was significantly down-regulated in comparison to the control group, but the expression of miRNA-21 and miR-30 significantly increased in the EXO and PBMT groups in comparison to the control group. The EXO and PBMT significantly increased GSH and nucleus acetylation (P<0.0001). Conclusion: The results of this study showed that the use of EXO and PBMT can improve GSH and nucleus acetylation in the PCOS oocyte and also change the expression of miRNAs.

Keywords: Exosome therapy; PCOS; Photobiomodulation therapy; miR-155; miR-21.