A novel method for measurements of sleep/wake states, feeding and drinking behaviors using the tracking technique of 3D positions in freely moving mice

Toshiyuki Hamada; Kenneth Sutherland; Masayori Ishikawa; Jun Saito; Naoki Miyamoto; Sato Honma; Hiroki Shirato; Ken-Ichi Honma

doi:10.1016/j.bbrc.2024.150359

A novel method for measurements of sleep/wake states, feeding and drinking behaviors using the tracking technique of 3D positions in freely moving mice

Biochem Biophys Res Commun. 2024 Nov 5:732:150359. doi: 10.1016/j.bbrc.2024.150359. Epub 2024 Jul 10.

Authors

Toshiyuki Hamada¹, Kenneth Sutherland², Masayori Ishikawa³, Jun Saito⁴, Naoki Miyamoto⁵, Sato Honma⁶, Hiroki Shirato², Ken-Ichi Honma⁶

Affiliations

¹ Department of Pharmaceutical Sciences, International University of Health and Welfare, Ohtawara, Tochigi, 324-8501, Japan. Electronic address: toshi-ha@iuhw.ac.jp.
² Global Center for Biomedical Science and Engineering, Hokkaido University, North 15 West 7, Kita-ku, Sapporo, 060-8638, Japan.
³ Department of Biomedical Science and Engineering, Faculty of Health Sciences, Hokkaido University, Sapporo, Hokkaido, 060-0812, Japan.
⁴ Department of Pharmaceutical Sciences, International University of Health and Welfare, Ohtawara, Tochigi, 324-8501, Japan.
⁵ Division of Applied Quantum Science and Engineering, Faculty of Engineering, Hokkaido University, North15 West 7, Kita-ku, Sapporo, 060-8638, Japan.
⁶ Department of Chronomedicine, Hokkaido University Graduate School of Medicine, Sapporo, Hokkaido, 060-8638, Japan.

PMID: 39032409
DOI: 10.1016/j.bbrc.2024.150359

Abstract

We have previously developed a 3D video tracking system which enables us to analyze long-term quantitative analysis of gene expression in freely moving mice. In the present study, we improved 3D video tracking and developed a system that analyzes more detailed behavioral data. We succeeded in simultaneously analyzing sleep-wake, feeding, and drinking behavior rhythms in the same individual using our tracking system. This system will make it possible to measure gene expression in each tissue in vivo in real time in relation to the various behavioral rhythms mentioned above.

Keywords: Bioluminescence; Circadian rhythm; Cry1; Cry2; D-luciferin; In vivo imaging; Period1.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Drinking Behavior / physiology
Feeding Behavior* / physiology
Imaging, Three-Dimensional / methods
Male
Mice
Mice, Inbred C57BL
Sleep* / physiology
Video Recording / methods
Wakefulness* / physiology