Antimicrobial peptide-fibrin glue mixture for treatment of methicillin-resistant Staphylococcus aureus-infected wounds

Ther Deliv. 2024;15(8):577-591. doi: 10.1080/20415990.2024.2369497. Epub 2024 Jul 16.

Abstract

Aim: This study was conducted to investigate the effect of fibrin glue-CM11 antibacterial peptide mixture (FG-P) on the healing of infected wounds in vivo.Materials & methods: We formulated a mixture of FG-P and evaluated its antimicrobial activity in vitro against multidrug-resistant (MDR) bacteria involved in wound infection as well as its healing effect on wound infected by methicillin-resistant S. aureus (MRSA) in vivo.Results: The peptide had an MIC of 8 μg/ml against all bacteria isolates. Growth inhibition zones were evident for FG-P compared with FG. The in vivo study showed that the FG-P could be significantly effective in healing the MRSA-infected wound.Conclusion: The use of FG-P mixture is a very suitable option for treating infected wounds.

Keywords: Staphylococcus aureus; antimicrobial peptide; fibrin glue; infection; wound healing.

Plain language summary

[Box: see text].

MeSH terms

  • Animals
  • Anti-Bacterial Agents / administration & dosage
  • Anti-Bacterial Agents / pharmacology
  • Antimicrobial Peptides / administration & dosage
  • Antimicrobial Peptides / chemistry
  • Antimicrobial Peptides / pharmacology
  • Fibrin Tissue Adhesive* / administration & dosage
  • Fibrin Tissue Adhesive* / pharmacology
  • Male
  • Methicillin-Resistant Staphylococcus aureus* / drug effects
  • Mice
  • Microbial Sensitivity Tests*
  • Staphylococcal Infections* / drug therapy
  • Staphylococcal Infections* / microbiology
  • Wound Healing* / drug effects
  • Wound Infection* / drug therapy
  • Wound Infection* / microbiology

Substances

  • Fibrin Tissue Adhesive
  • Anti-Bacterial Agents
  • Antimicrobial Peptides