Butyrate induces higher host transcriptional changes to inhibit porcine epidemic diarrhea virus strain CV777 infection in porcine intestine epithelial cells

Virol J. 2024 Jul 11;21(1):157. doi: 10.1186/s12985-024-02428-5.

Abstract

Newborn piglets' health is seriously threatened by the porcine epidemic diarrhea virus (PEDV), which also has a significant effect on the pig industry. The gut microbiota produces butyrate, an abundant metabolite that modulates intestinal function through many methods to improve immunological and intestinal barrier function. The objective of this investigation was to ascertain how elevated butyrate concentrations impacted the host transcriptional profile of PEDV CV777 strain infection. Our findings showed that higher concentrations of butyrate have a stronger inhibitory effect on PEDV CV777 strain infection. According to RNA-seq data, higher concentrations of butyrate induced more significant transcriptional changes in IPEC-J2 cells, and signaling pathways such as PI3K-AKT may play a role in the inhibition of PEDV CV777 strain by high concentrations of butyrate. Ultimately, we offer a theoretical and experimental framework for future research and development of novel approaches to harness butyrate's antiviral infection properties.

Keywords: Butyrate; Pigs; Porcine epidemic diarrhea virus; RNA-seq.

MeSH terms

  • Animals
  • Antiviral Agents / pharmacology
  • Butyrates* / metabolism
  • Butyrates* / pharmacology
  • Cell Line
  • Coronavirus Infections / drug therapy
  • Coronavirus Infections / veterinary
  • Coronavirus Infections / virology
  • Epithelial Cells* / drug effects
  • Epithelial Cells* / virology
  • Intestinal Mucosa / drug effects
  • Intestinal Mucosa / metabolism
  • Intestinal Mucosa / virology
  • Intestines / virology
  • Porcine epidemic diarrhea virus* / drug effects
  • Porcine epidemic diarrhea virus* / physiology
  • Signal Transduction / drug effects
  • Swine
  • Swine Diseases / virology
  • Virus Replication / drug effects

Substances

  • Butyrates
  • Antiviral Agents