Anti-inflammation mechanisms of a homogeneous polysaccharide from Phyllanthus emblica L. on DSS induced colitis mice via the gut microbiota and metabolites alteration

Xiaoqing Li; Yihan Chen; Xinan Peng; Yi Zhu; Wen Duan; Ruya Ji; Hang Xiao; Xueli Li; Guo Liu; Yigang Yu; Yong Cao

doi:10.1016/j.foodchem.2024.140346

Anti-inflammation mechanisms of a homogeneous polysaccharide from Phyllanthus emblica L. on DSS induced colitis mice via the gut microbiota and metabolites alteration

Food Chem. 2024 Nov 30:459:140346. doi: 10.1016/j.foodchem.2024.140346. Epub 2024 Jul 4.

Authors

Xiaoqing Li¹, Yihan Chen², Xinan Peng³, Yi Zhu³, Wen Duan⁴, Ruya Ji⁵, Hang Xiao⁵, Xueli Li⁶, Guo Liu⁷, Yigang Yu⁸, Yong Cao⁹

Affiliations

¹ Guangdong Provincial Key Laboratory of Nutraceuticals and Functional Foods, College of Food Science, South China Agricultural University, Guangzhou, 510642, China; College of Food Science and Engineering, South China University of Technology, Guangzhou 510006, China,; Department of Food Science, University of Massachusetts, Amherst, MA 01003, USA.
² State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi 214122, China.
³ Guangdong Provincial Key Laboratory of Nutraceuticals and Functional Foods, College of Food Science, South China Agricultural University, Guangzhou, 510642, China.
⁴ College of Food Science and Engineering, South China University of Technology, Guangzhou 510006, China.
⁵ Department of Food Science, University of Massachusetts, Amherst, MA 01003, USA.
⁶ Eastroc Beverage Group Co., Ltd., Shenzhen, 518057, China.
⁷ College of Light Industry and Food, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong, 510225, China. Electronic address: liuguo@zhku.edu.cn.
⁸ College of Food Science and Engineering, South China University of Technology, Guangzhou 510006, China,. Electronic address: yuyigang@scut.edu.cn.
⁹ Guangdong Provincial Key Laboratory of Nutraceuticals and Functional Foods, College of Food Science, South China Agricultural University, Guangzhou, 510642, China. Electronic address: Caoyong2181@scau.edu.cn.

PMID: 38981378
DOI: 10.1016/j.foodchem.2024.140346

Abstract

Phyllanthus emblica L. offers promising therapeutic potential for inflammatory diseases. This study revealed the molecular structure of a homogeneous polysaccharide purified from Phyllanthus emblica L. (PEP-1) and evaluated its anti-inflammatory effects on ulcerative colitis (UC) in mice. In the in vivo experiment, administered in varying dosages to dextran sulfate sodium (DSS)-induced UC models, PEP-1 significantly alleviated colonic symptoms, histological damages and reshaped the gut microbiota. Notably, it adjusted the Firmicutes/Bacteroidetes ratio and reduced pro-inflammatory species, closely aligning with shifts in the fecal metabolites and metabolic pathways such as the metabolism of pyrimidine, beta-alanine, and purine. These findings underscore the potential of PEP-1 as a therapeutic agent for UC, providing insights into the mechanisms through gut microbiota and metabolic modulation.

Keywords: Colonic inflammation; DSS; Gut microbiome; Metabolomic profile; Phyllanthus emblica L. polysaccharide; Structure.

MeSH terms

Animals
Anti-Inflammatory Agents* / administration & dosage
Anti-Inflammatory Agents* / chemistry
Anti-Inflammatory Agents* / pharmacology
Bacteria* / classification
Bacteria* / drug effects
Bacteria* / genetics
Bacteria* / isolation & purification
Colitis / chemically induced
Colitis / drug therapy
Colitis / metabolism
Colitis / microbiology
Colitis, Ulcerative* / chemically induced
Colitis, Ulcerative* / drug therapy
Colitis, Ulcerative* / metabolism
Colitis, Ulcerative* / microbiology
Colon / drug effects
Colon / immunology
Colon / metabolism
Colon / microbiology
Dextran Sulfate* / adverse effects
Disease Models, Animal
Gastrointestinal Microbiome* / drug effects
Humans
Male
Mice
Mice, Inbred C57BL
Phyllanthus emblica* / chemistry
Plant Extracts* / administration & dosage
Plant Extracts* / chemistry
Plant Extracts* / pharmacology
Polysaccharides* / administration & dosage
Polysaccharides* / chemistry
Polysaccharides* / isolation & purification
Polysaccharides* / pharmacology

Substances

Dextran Sulfate
Polysaccharides
Anti-Inflammatory Agents
Plant Extracts