In-vitro and in-vivo investigation of wound healing efficacy of Syzygium cumini leaf extracts loaded carboxymethylcellulose film

C Balaji Ayyanar; Trishna Bal; Fahaduddin; Shreya Sharma; B Gayathri; V Rinusuba; H Esther Nalini; S Deepa; M Dhivyya Dharshinii; Pankaj Kharra; Aditi Sinha

doi:10.1016/j.ijbiomac.2024.133691

In-vitro and in-vivo investigation of wound healing efficacy of Syzygium cumini leaf extracts loaded carboxymethylcellulose film

Int J Biol Macromol. 2024 Aug;275(Pt 2):133691. doi: 10.1016/j.ijbiomac.2024.133691. Epub 2024 Jul 5.

Authors

C Balaji Ayyanar¹, Trishna Bal², Fahaduddin³, Shreya Sharma³, B Gayathri⁴, V Rinusuba⁴, H Esther Nalini⁵, S Deepa⁶, M Dhivyya Dharshinii⁷, Pankaj Kharra⁸, Aditi Sinha⁹

Affiliations

¹ Department of Mechanical Engineering, Coimbatore Institute of Technology, Coimbatore 641014, Tamil Nadu, India. Electronic address: balajiayyanar2007@gmail.com.
² Department of Pharmaceutical Sciences & Technology, Birla Institute of Technology, Mesra, Ranchi 835215, India. Electronic address: trishna.bal@gmail.com.
³ Department of Pharmaceutical Sciences & Technology, Birla Institute of Technology, Mesra, Ranchi 835215, India.
⁴ Department of Chemistry, Coimbatore Institute of Technology, Coimbatore, Tamil Nadu, India.
⁵ Department of Periodontics KSR Institute of Dental Science and Research, Tiruchengode, Namakkal, Tamil Nadu, India.
⁶ Department of Prosthodontics, RVS Dental College and Hospital, Kumaran Kottam Campus, Kannampalayam, Coimbatore, Tamil Nadu 641402, India.
⁷ Department of Electrical and Electronics Engineering, Rajalakshmi Engineering College Thandalam, Chennai 602 105, India.
⁸ Institute of Nano Science and Technology, Habitat Centre, Phase-10, Sector-64, Mohali, Punjab 160062, India.
⁹ JSS College of Pharmacy, Mysore, India.

PMID: 38972647
DOI: 10.1016/j.ijbiomac.2024.133691

Abstract

The study focused on Syzygium cumini Leaf Extract (SCLE) loaded into Carboxymethylcellulose (CMC) film via Solution casting. Phytochemical screening revealed carbohydrates, and HPLC analysis identified quercetin, known for promoting wound healing. FT-IR spectroscopy confirmed various functional groups. X-Ray diffraction (XRD) determined the crystallite size to be 14.58 nm. Field Emission Scanning Electron Microscopy (FESEM) showed the dispersion of extracts, and Energy Dispersive X-ray (EDX) analysis detailed the weight percentages of components. Antibacterial activity tests revealed zones of inhibition for S. aureus (15 mm) and E. coli (11 mm). The film exhibited 63.11 % antioxidant activity at 517 nm with DPPH at a 750 μl sample concentration. Drug release kinetics were also studied. In-vitro wound healing using the L929 cell line showed 83 % healing at a 100 μl concentration. Over 14 days, the treatment group's wounds healed completely within 7 days, unlike the control groups which showed no recovery after 14 days. These findings indicate that the SCLE-CMC film is highly effective in promoting wound healing.

Keywords: Carboxymethylcellulose; In-vitro wound healing; Jamun leaf.

MeSH terms

Animals
Anti-Bacterial Agents* / chemistry
Anti-Bacterial Agents* / pharmacology
Antioxidants* / chemistry
Antioxidants* / pharmacology
Carboxymethylcellulose Sodium* / chemistry
Carboxymethylcellulose Sodium* / pharmacology
Cell Line
Drug Liberation
Escherichia coli / drug effects
Male
Mice
Plant Extracts* / chemistry
Plant Extracts* / pharmacology
Plant Leaves* / chemistry
Rats
Staphylococcus aureus / drug effects
Syzygium* / chemistry
Wound Healing* / drug effects

Substances

Carboxymethylcellulose Sodium
Plant Extracts
Anti-Bacterial Agents
Antioxidants