Copper nitroprusside analogue nanoparticles against melanoma: detailed in vitro and in vivo investigation

Nanoscale. 2024 Jul 18;16(28):13580-13596. doi: 10.1039/d4nr01857e.

Abstract

Melanoma is the most invasive and lethal form of skin cancer that arises from the malignant transformation of specialized pigment-producing cell melanocytes. Nanomedicine represents an important prospect to mitigate the difficulties and provide significant benefits to cure melanoma. In the present study, we investigated in vitro and in vivo therapeutic efficacies of copper nitroprusside analogue nanoparticles (abbreviated as CuNPANP) towards melanoma. Initially, in vitro anti-cancer activities of CuNPANP towards melanoma cells (B16F10) were evaluated by several experiments such as [methyl-3H]-thymidine incorporation assay, cell cycle and apoptosis assays using FACS analysis, ROS generation using DCFDA, DHE and DAF2A reagents, internalization of nanoparticles through ICP-OES analysis, co-localization of the nanoparticles using confocal microscopy, JC-1 staining to investigate the mitochondrial membrane potential (MMP) and immunofluorescence studies to analyze the expressions of cytochrome-c, Ki-67, E-cadherin as well as phalloidin staining to analyze the cytoskeletal integrity. Further, the in vivo therapeutic effectiveness of the nanoparticles was established towards malignant melanoma by inoculating B16F10 cells in the dorsal right abdomen of C57BL/6J mice. The intraperitoneal administration of CuNPANP inhibited tumor growth and increased the survivability of melanoma mice. The in vivo immunofluorescence studies (Ki-67, CD-31, and E-cadherin) and TUNEL assay further support the anti-cancer and apoptosis-inducing potential of CuNPANP, respectively. Finally, various signaling pathways and molecular mechanisms involved in anti-cancer activities were further evaluated by Western blot analysis. The results altogether indicated the potential use of copper-based nanomedicines for the treatment of malignant melanoma.

MeSH terms

  • Animals
  • Antineoplastic Agents / chemistry
  • Antineoplastic Agents / pharmacology
  • Apoptosis* / drug effects
  • Cell Line, Tumor
  • Cell Proliferation / drug effects
  • Copper* / chemistry
  • Copper* / pharmacology
  • Melanoma / drug therapy
  • Melanoma / metabolism
  • Melanoma / pathology
  • Melanoma, Experimental* / drug therapy
  • Melanoma, Experimental* / metabolism
  • Melanoma, Experimental* / pathology
  • Membrane Potential, Mitochondrial / drug effects
  • Metal Nanoparticles / chemistry
  • Metal Nanoparticles / therapeutic use
  • Mice
  • Mice, Inbred C57BL*
  • Nanoparticles / chemistry
  • Nitroprusside* / chemistry
  • Nitroprusside* / pharmacology
  • Reactive Oxygen Species / metabolism
  • Skin Neoplasms / drug therapy
  • Skin Neoplasms / metabolism
  • Skin Neoplasms / pathology

Substances

  • Copper
  • Nitroprusside
  • Reactive Oxygen Species
  • Antineoplastic Agents