Functional evaluation of pure natural edible Ferment: protective function on ulcerative colitis

Yanjun Chen; Shengzhi Ye; Jiaolong Shi; Hao Wang; Guangxu Deng; Guangxian Wang; Shijie Wang; Qingbin Yuan; Lunan Yang; Tingyu Mou

doi:10.3389/fmicb.2024.1367630

Functional evaluation of pure natural edible Ferment: protective function on ulcerative colitis

Front Microbiol. 2024 Jun 17:15:1367630. doi: 10.3389/fmicb.2024.1367630. eCollection 2024.

Authors

Yanjun Chen^#¹, Shengzhi Ye^#¹, Jiaolong Shi^#¹, Hao Wang², Guangxu Deng³, Guangxian Wang⁴, Shijie Wang^{5

6}, Qingbin Yuan⁶, Lunan Yang⁷, Tingyu Mou¹

Affiliations

¹ Department of General Surgery & Guangdong Provincial Key Laboratory of Precision Medicine for Gastrointestinal Cancer, Nanfang Hospital, Southern Medical University, Guangzhou, China.
² First Department of Gastrointestinal Surgery, Hainan General Hospital, Hainan Affiliated Hospital of Hainan Medical University, Haikou, China.
³ Department of Gastrointestinal and Anorectal, The First People's Hospital of Zhaoqing, Zhaoqing, China.
⁴ Jiangsu Biodep Biotechnology Co., Ltd., Jiangyin, China.
⁵ College of Foods Science and Biology, Hebei University of Science and Technology, Shijiazhuang, China.
⁶ Junlebao Dairy Group Co., Ltd., Shijiazhuang, China.
⁷ Department of Plastic and Aesthetic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, China.

^# Contributed equally.

Abstract

Purpose: To investigate the therapeutic efficiency of a novel drink termed "Ferment" in cases of ulcerative colitis (UC) and its influence on the gut microbiota.

Method: In this study, we developed a complex of mixed fruit juice and lactic acid bacteria referred to as Ferment. Ferment was fed to mice for 35 days, before inducing UC with Dextran Sulfate Sodium Salt. We subsequently investigated the gut microbiome composition using 16S rRNA sequencing.

Result: After Ferment treatment, mouse body weight increased, and animals displayed less diarrhea, reduced frequency of bloody stools, and reduced inflammation in the colon. Beneficial bacteria belonging to Ileibacterium, Akkermansia, and Prevotellacea were enriched in the gut after Ferment treatment, while detrimental organisms including Erysipelatoclostridium, Dubosiella, and Alistipes were reduced.

Conclusion: These data place Ferment as a promising dietary candidate for enhancing immunity and protecting against UC.

Keywords: 16S rRNA; Ferment; animal experiment; lactic acid bacteria; ulcerative colitis (UC).

Grants and funding

The author(s) declare that financial support was received for the research, authorship, and/or publication of this article. This work was supported by grants from the Guangdong Provincial Key Laboratory of Precision Medicine for Gastrointestinal Cancer (2020B121201004), Natural Science Foundation, Regional Joint Fund of Guangdong Province of China (2020A1515110140), Guangdong Provincial Natural Science Foundation (2023A1515010276) and the Key Research and Development Program of Hebei (No. 19227128D) and (No. 21327110D) Science and Technology Programme of Shijiazhuang (07202202 & 228790859A).