PSSR2 improves and expands on the previously established PSSR (Point-Scanning Super-Resolution) workflow for simultaneous super-resolution and denoising of undersampled microscopy data. PSSR2 is designed to put state-of-the-art technology into the hands of the general microscopy and biology research community, enabling user-friendly implementation of PSSR workflows with little to no programming experience required, especially through its integrated CLI and Napari plugin.