A new FRET-based fluorescent probe: Colorimetric and ratiometric detection of hypochlorite and anti-counterfeiting applications

Spectrochim Acta A Mol Biomol Spectrosc. 2024 Oct 5:318:124477. doi: 10.1016/j.saa.2024.124477. Epub 2024 May 17.

Abstract

Hypochlorite (ClO-), as the main component of widely used disinfectants in daily life, comes into closer contact with the human body, which can lead to a number of diseases. The high-performance method is increasingly needed to detect ClO- in our daily life. In this report, we successfully synthesized a FRET ratiometric fluorescent probe (NDAC) containing benzoxadiazole moieties and coumarin moieties bound via ethylenediamine. As expected, NDAC has excellent selectivity and anti-interference ability toward ClO-, and the ratio of fluorescence intensity (I471 nm/I533 nm) has a very good linear relationship with the concentration of ClO-, with a wide linear range (2.5-1750 μM) and low detection limit (0.887 μM). Furthermore, we have successfully applied it for the quantitative detection of ClO- in water samples in daily life. At the same time, there is a very clear change in the fluorescence color after the reaction of the NDAC with ClO-. The blue/green value (B/G) of this color change also shows a very good linear relationship to ClO- (5.0-1000 μM). Therefore, the NDAC has also been successfully used for test strip detection and quantitative detection of ClO- in actual samples through smartphone-based fluorescence image analysis, and this method can provide faster, more convenient and more accessible detection. In addition, NDAC sensors also have potential applications in the field of information anti-counterfeiting.

Keywords: FRET; Hypochlorite; Ratiometric; Smartphone imaging.

MeSH terms

  • Colorimetry* / methods
  • Coumarins / chemistry
  • Disinfectants / analysis
  • Fluorescence Resonance Energy Transfer* / methods
  • Fluorescent Dyes* / chemical synthesis
  • Fluorescent Dyes* / chemistry
  • Humans
  • Hypochlorous Acid* / analysis
  • Limit of Detection

Substances

  • Fluorescent Dyes
  • Hypochlorous Acid
  • Disinfectants
  • Coumarins