The InBIO Barcoding Initiative Database: DNA barcodes of Orthoptera from Portugal

Sílvia Pina; Joana Pauperio; Francisco Barros; Cátia Chaves; Filipa Ms Martins; Joana Pinto; Joana Veríssimo; Vanessa A Mata; Pedro Beja; Sónia Ferreira

doi:10.3897/BDJ.12.e118010

The InBIO Barcoding Initiative Database: DNA barcodes of Orthoptera from Portugal

Biodivers Data J. 2024 May 15:12:e118010. doi: 10.3897/BDJ.12.e118010. eCollection 2024.

Authors

Sílvia Pina^{1

2

3}, Joana Pauperio^{1

4}, Francisco Barros⁵, Cátia Chaves^{1

2}, Filipa Ms Martins^{1

2}, Joana Pinto^{1

2}, Joana Veríssimo^{1

2}, Vanessa A Mata^{1

2}, Pedro Beja^{1

2

3}, Sónia Ferreira^{1

2

6}

Affiliations

¹ CIBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, InBIO Laboratório Associado, Campus de Vairão, Universidade do Porto, 4485-661 Vairão, Vila do Conde, Portugal CIBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, InBIO Laboratório Associado, Campus de Vairão, Universidade do Porto, 4485-661 Vairão Vila do Conde Portugal.
² BIOPOLIS Program in Genomics, Biodiversity and Land Planning, CIBIO, Campus de Vairão, 4485-661 Vairão, Vila do Conde, Portugal BIOPOLIS Program in Genomics, Biodiversity and Land Planning, CIBIO, Campus de Vairão, 4485-661 Vairão Vila do Conde Portugal.
³ CIBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, InBIO Laboratório Associado, Instituto Superior de Agronomia, Universidade de Lisboa, Lisboa, Portugal CIBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, InBIO Laboratório Associado, Instituto Superior de Agronomia, Universidade de Lisboa Lisboa Portugal.
⁴ European Molecular Biology Laboratory, European Bioinformatics Institute, Wellcome Genome Campus, Hinxton, Cambridge, United Kingdom European Molecular Biology Laboratory, European Bioinformatics Institute Wellcome Genome Campus, Hinxton, Cambridge United Kingdom.
⁵ Rua da Eira 3, S. Salvador, 2550-251, Cercal, Portugal Rua da Eira 3, S. Salvador, 2550-251 Cercal Portugal.
⁶ EBM, Estação Biológica de Mertola, Praça Luis de Camoes, Mertola, Mertola, Portugal EBM, Estação Biológica de Mertola, Praça Luis de Camoes, Mertola Mertola Portugal.

Abstract

Background: The InBIO Barcoding Initiative (IBI) Orthoptera dataset contains records of 420 specimens covering all the eleven Orthoptera families occurring in Portugal. Specimens were collected in continental Portugal from 2005 to 2021 and were morphologically identified to species level by taxonomists. A total of 119 species were identified corresponding to about 77% of all the orthopteran species known from continental Portugal.

New information: DNA barcodes of 54 taxa were made public for the first time at the Barcode of Life Data System (BOLD). Furthermore, the submitted sequences were found to cluster in 129 BINs (Barcode Index Numbers), 35 of which were new additions to the Barcode of Life Data System (BOLD). All specimens have their DNA barcodes publicly accessible through BOLD online database. Stenobothruslineatus is recorded for the first time for continental Portugal. This dataset greatly increases the knowledge on the DNA barcodes and distribution of Orthoptera from Portugal. All DNA extractions and most specimens are deposited in the IBI collection at CIBIO, Research Center in Biodiversity and Genetic Resources.

Keywords: DNA barcode; Orthoptera; continental Portugal; cytochrome c oxidase subunit I (COI); species distributions.

Sílvia Pina, Joana Pauperio, Francisco Barros, Cátia Chaves, Filipa MS Martins, Joana Pinto, Joana Veríssimo, Vanessa A Mata, Pedro Beja, Sónia Ferreira.