A detection system using sensing motif-tethered oligodeoxynucleotides for multiplex biomolecular analysis

Tatsuya Nishihara; Yuto Motohashi; Reoto Mio; Masato Sugawara; Kazuhito Tanabe

doi:10.1039/d4cc01470g

A detection system using sensing motif-tethered oligodeoxynucleotides for multiplex biomolecular analysis

Chem Commun (Camb). 2024 Jun 6;60(47):6059-6062. doi: 10.1039/d4cc01470g.

Authors

Tatsuya Nishihara¹, Yuto Motohashi¹, Reoto Mio¹, Masato Sugawara¹, Kazuhito Tanabe¹

Affiliation

¹ Department of Chemistry and Biological Science, College of Science and Engineering, Aoyama Gakuin University, 5-10-1 Fuchinobe, Chuo-ku, Sagamihara 252-5258, Japan. nishihara@chem.aoyama.ac.jp.

PMID: 38780054
DOI: 10.1039/d4cc01470g

Abstract

We developed a system to detect multiple target biomolecules through sensing motif-tethered oligodeoxynucleotides. DNA-based molecular probes gave the primary amine motif upon reaction with the target biomolecules, glutathione (GSH) and H₂O₂. After labelling with biotin, the product DNAs were selectively collected to be quantified by qPCR.

MeSH terms

Biosensing Techniques / methods
Biotin* / chemistry
DNA / chemistry
Glutathione* / analysis
Glutathione* / chemistry
Hydrogen Peroxide* / analysis
Hydrogen Peroxide* / chemistry
Oligodeoxyribonucleotides* / chemistry
Oligodeoxyribonucleotides* / genetics

Substances

Oligodeoxyribonucleotides
Hydrogen Peroxide
Glutathione
Biotin
DNA