Identification of Mycobacterium abscessus using the peaks of ribosomal protein L29, L30 and hemophore-related protein by MALDI-MS proteotyping

Satomi Takei; Kanae Teramoto; Yuji Sekiguchi; Hiroaki Ihara; Mari Tohya; Shinichi Iwamoto; Koichi Tanaka; Abdullah Khasawneh; Yuki Horiuchi; Shigeki Misawa; Toshio Naito; Teruo Kirikae; Tatsuya Tada; Yoko Tabe

doi:10.1038/s41598-024-61549-7

Identification of Mycobacterium abscessus using the peaks of ribosomal protein L29, L30 and hemophore-related protein by MALDI-MS proteotyping

Sci Rep. 2024 May 16;14(1):11187. doi: 10.1038/s41598-024-61549-7.

Authors

Satomi Takei^{1

2}, Kanae Teramoto^{2

3}, Yuji Sekiguchi⁴, Hiroaki Ihara^{2

5}, Mari Tohya⁶, Shinichi Iwamoto⁷, Koichi Tanaka⁷, Abdullah Khasawneh¹, Yuki Horiuchi¹, Shigeki Misawa^{2

8}, Toshio Naito^{2

9}, Teruo Kirikae^{2

10}, Tatsuya Tada¹¹, Yoko Tabe^{1

2}

Affiliations

¹ Department of Clinical Laboratory Medicine, Juntendo University Graduate School of Medicine, Tokyo, Japan.
² Department of MALDI-TOF MS Practical Application Research, Juntendo University Graduate School of Medicine, Tokyo, Japan.
³ Analytical and Measurement Instruments Division, Shimadzu Corporation, Kyoto, Japan.
⁴ Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, Japan.
⁵ Department of Respiratory Medicine, Juntendo University Graduate School of Medicine, Tokyo, Japan.
⁶ Department of Microbiology, Juntendo University Graduate School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo, 113-8421, Japan.
⁷ Koichi Tanaka Mass Spectrometry Research Laboratory, Shimadzu Corporation, Kyoto, Japan.
⁸ Department of Clinical Laboratory Technology, Faculty of Medical Science, Juntendo University, Tokyo, Japan.
⁹ Department of General Medicine, Juntendo University Graduate School of Medicine, Tokyo, Japan.
¹⁰ Department of Microbiome Research, Juntendo University Graduate School of Medicine, Tokyo, Japan.
¹¹ Department of Microbiology, Juntendo University Graduate School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo, 113-8421, Japan. t-tada@juntendo.ac.jp.

Abstract

Mycobacteroides (Mycobacterium) abscessus, which causes a variety of infectious diseases in humans, is becoming detected more frequently in clinical specimens as cases are spreading worldwide. Taxonomically, M. abscessus is composed of three subspecies of M. abscessus subsp. abscessus, M. abscessus subsp. bolletii, and M. abscessus subsp. massiliense, with different susceptibilities to macrolides. In order to identify rapidly these three subspecies, we determined useful biomarker proteins, including ribosomal protein L29, L30, and hemophore-related protein, for distinguishing the subspecies of M. abscessus using the matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) profiles. Thirty-three clinical strains of M. abscessus were correctly identified at the subspecies-level by the three biomarker protein peaks. This study ultimately demonstrates the potential of routine MALDI-MS-based laboratory methods for early identification and treatment for M. abscessus infections.

MeSH terms

Bacterial Proteins* / metabolism
Biomarkers / analysis
Biomarkers / metabolism
Humans
Mycobacterium Infections, Nontuberculous / diagnosis
Mycobacterium Infections, Nontuberculous / microbiology
Mycobacterium abscessus* / metabolism
Ribosomal Proteins* / analysis
Ribosomal Proteins* / metabolism
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization* / methods

Abstract

MeSH terms

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