Gallic acid attenuates torsion/detorsion-induced testicular injury in rats through suppressing of HMGB1/NF-κB axis and endoplasmic reticulum stress

Rev Int Androl. 2024 Mar;22(1):1-7. doi: 10.22514/j.androl.2024.001. Epub 2024 Mar 30.

Abstract

It was aimed to evaluate whether gallic acid (GA) have a beneficial effect in the testicular ischemia/reperfusion injury (IRI) model in rats for the first time. Testicular malondialdehyde, 8-hydroxy-2'-deoxyguanosine, superoxide dismutase, catalase, high mobility group box 1 protein, nuclear factor kappa B, tumor necrosis factoralpha, interleukin-6, myeloperoxidase, 78-kDa glucose-regulated protein, activating transcription factor 6, CCAAT-enhancer-binding protein homologous protein and caspase-3 levels were determined using colorimetric methods. The oxidative stress, inflammation, endoplasmic reticulum stress and apoptosis levels increased statistically significantly in the IRI group compared with the sham operated group (p < 0.05). GA application improved these damage significantly (p < 0.05). Moreover, it was found that the results of histological examinations supported the biochemical results to a statistically significant extent. Our findings suggested that GA may be evaluated as a protective agent against testicular IRI.

Keywords: Apoptosis; Endoplasmic reticulum stress; Gallic acid; Inflammation; Oxidative stress; Testicular torsion.

MeSH terms

  • Animals
  • Apoptosis / drug effects
  • Endoplasmic Reticulum Stress* / drug effects
  • Gallic Acid* / administration & dosage
  • Gallic Acid* / pharmacology
  • HMGB1 Protein* / drug effects
  • HMGB1 Protein* / metabolism
  • Male
  • NF-kappa B* / drug effects
  • NF-kappa B* / metabolism
  • Oxidative Stress* / drug effects
  • Rats
  • Rats, Sprague-Dawley
  • Reperfusion Injury* / drug therapy
  • Reperfusion Injury* / metabolism
  • Reperfusion Injury* / prevention & control
  • Spermatic Cord Torsion* / drug therapy
  • Testis / drug effects
  • Testis / metabolism
  • Testis / pathology

Substances

  • Gallic Acid
  • Hbp1 protein, rat
  • HMGB1 Protein
  • NF-kappa B