Annotation of nuclear lncRNAs based on chromatin interactions

PLoS One. 2024 May 6;19(5):e0295971. doi: 10.1371/journal.pone.0295971. eCollection 2024.

Abstract

The human genome is pervasively transcribed and produces a wide variety of long non-coding RNAs (lncRNAs), constituting the majority of transcripts across human cell types. Some specific nuclear lncRNAs have been shown to be important regulatory components acting locally. As RNA-chromatin interaction and Hi-C chromatin conformation data showed that chromatin interactions of nuclear lncRNAs are determined by the local chromatin 3D conformation, we used Hi-C data to identify potential target genes of lncRNAs. RNA-protein interaction data suggested that nuclear lncRNAs act as scaffolds to recruit regulatory proteins to target promoters and enhancers. Nuclear lncRNAs may therefore play a role in directing regulatory factors to locations spatially close to the lncRNA gene. We provide the analysis results through an interactive visualization web portal at https://fantom.gsc.riken.jp/zenbu/reports/#F6_3D_lncRNA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Nucleus / genetics
  • Cell Nucleus / metabolism
  • Chromatin* / genetics
  • Chromatin* / metabolism
  • Genome, Human
  • Humans
  • Molecular Sequence Annotation
  • Promoter Regions, Genetic
  • RNA, Long Noncoding* / genetics
  • RNA, Long Noncoding* / metabolism

Substances

  • RNA, Long Noncoding
  • Chromatin

Grants and funding

FANTOM6 was made possible by a Research Grant for RIKEN Center for Life Science Technologies, Division of Genomic Technologies (CLST DGT) and RIKEN Center for Integrative Medical Sciences (IMS) from the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan. This work was supported by the Natural Sciences and Engineering Research Council of Canada (RGPIN-2015-03948 to M.M.H.). I.V.K., A.B. and V.J.M. were supported by the Ministry of Science and Higher Education of the Russian Federation (Grant No. 075-15-2021-601). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.